-
Plasmid#185895PurposeIn vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).DepositorInsertPRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PSk.GAL2>YFAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 25, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTre-Tight-luciferase-GFP (467)
Plasmid#65491Purposenegative control for expression assaysDepositorInsertEGFP
UseLuciferaseTagsExpressionMammalianMutationPromotertight TRE promoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-GFPsv40 GGGG AAGA
Plasmid#174531Purpose3xP3-GFP fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-GFP BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3_HES4
Plasmid#124072PurposeHES4 with normal stop codonDepositorInsertHES4 (HES4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceApril 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti_PGK_Prkaa1
Plasmid#204356PurposeLentiviral plasmid, Expresses Prkaa1 under the control of the PGK promoterDepositorInsertAMP-activated protein kinase alpha 1 (Prkaa1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_Efla_FLEX_APEX2-eGFPf
Plasmid#171939PurposeExpresses a membrane targeted (farnesylation) APEX2-eGFP fusion proteinDepositorInsertAPEX2-eGFPf
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEf1aAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMC4_OE_1
Plasmid#161652PurposeOverexpresses TMC4DepositorInsertTMC4 (TMC4 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-N1
Plasmid#54507PurposeLocalization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsPA-mCherry1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti-EF1a-GFP-miRE_shRen-Puro
Plasmid#135683PurposeConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selectionDepositorInsertshRenilla
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS1-his-24::tagBFP2
Plasmid#124321PurposeExpressing Nuclear Localized tagBFP2DepositorInserttagBFP2 (his-24 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-SpCas9-P2A-blast
Plasmid#106477Purposesingle-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screensDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pShuttle
Plasmid#67964PurposeMobilizible plasmid with EGFP. OriT from the F plasmid.DepositorInsertOriT
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
IDG_TMEM63C_OE_1
Plasmid#161695PurposeOverexpresses TMEM63CDepositorInsertTMEM63C (TMEM63C Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJuly 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
HT7-mTurquoise2-KDEL
Plasmid#188571PurposeExpresses fluorescent HaloTag7-mTurquoise2 fusion protein in mammalian cells with localization in the endoplasmic reticulum.DepositorInsertHaloTag7
UseTagsKDEL and mTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKK1 -pFlp-18::mito4x-GCaMP6f
Plasmid#194969PurposeFluorescent reporter of mitochondrial matrix Ca2+.DepositorInsert4 repeats of Cox8 presequence plus GCaMP6f
UseTagsExpressionWormMutationPromoterFlp-18Available sinceJune 21, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLIX403-ccdB-NatMx
Plasmid#158563PurposeSet of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusionsDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-EMCV_3C
Plasmid#201941PurposeExpresses EMCV 3C protease from a GAL promoter with a URA3 markerDepositorInsertEMCV 3C protease (EMCVgp1 )
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRARB-donor
Plasmid#132517PurposeCRISPR donor plasmid to create GFP fusion proteinsDepositorInsertRARB (RARB Human)
UseCRISPRTagsGFPExpressionMutationnonePromoterAvailable sinceMarch 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-syn-NES-jGCaMP8s-WPRE
Plasmid#186037PurposeAAV-mediated cytosolic expression of jGCaMP8f under the synapsin promoterDepositorInsertNES-jGCaMP8s
UseAAVTagsNES-6xHisExpressionMammalianMutationPromotersynapsinAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExp-NusA
Plasmid#112571PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and NusA fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8His and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only