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pnYC-CvH-Hs4EHP_1-234-I85AM101A_AE

Plasmid

#148562

Purpose

Bacterial Expression of Hs4EHP_1-234-I85AM101A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-I85AM101A (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_1-234-W95A_AE

Plasmid

#148563

Purpose

Bacterial Expression of Hs4EHP_1-234-W95A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_1-234-W95A (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-CvH-Hs4EHP_52-234opt-R103LE149L_AE

Plasmid

#148565

Purpose

Bacterial Expression of Hs4EHP_52-234opt-R103LE149L

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-R103LE149L (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnYC-NpH-Hs4EHP_52-234opt-Q159SM161DR202E_AE

Plasmid

#148567

Purpose

Bacterial Expression of Hs4EHP_52-234opt-Q159SM161DR202E

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP_52-234opt-Q159SM161DR202E (EIF4E2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4-del377-402_AG

Plasmid

#148759

Purpose

Mammalian Expression of HsNot4-del377-402

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del377-402 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot4-del400-428_AG

Plasmid

#148761

Purpose

Mammalian Expression of HsNot4-del400-428

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4-del400-428 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_1-274_AG

Plasmid

#148764

Purpose

Mammalian Expression of HsNot4_1-274

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_1-274 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_64-104-GSSG-275-575_AG

Plasmid

#148765

Purpose

Mammalian Expression of HsNot4_64-104-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_64-104-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-V5-SBP-MBP-HsNot4_109-189-GSSG-275-575_AG

Plasmid

#148767

Purpose

Mammalian Expression of HsNot4_109-189-GSSG_275-575

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

HsNot4_109-189-GSSG_275-575 (CNOT4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpM-HsGIGYF1_33-103-E44AE45FQ87A-GB1_AE

Plasmid

#148556

Purpose

Bacterial Expression of HsGIGYF1_33-103-E44AE45FQ87A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF1_33-103-E44AE45FQ87A (GIGYF1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNAres_AC

Plasmid

#148404

Purpose

Insect Expression of DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1-FNKRNFKFEtoAAAAAAAAA-dsRNares (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot4-dsRNAres_Q

Plasmid

#147339

Purpose

Insect Expression of DmNot4-dsRNAres

Depositor

Elisa Izaurralde

Article

Keskeny et al Genes Dev. 2019 Feb 1;33(3-4):236-252. d

Insert

DmNot4-dsRNAres (CG31716 Fly)

Use

Tags

Expression

Insect

Mutation

7 silent mutations and 5 non silent mutations G20…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-ε

Plasmid

#197261

Purpose

Expression of GAL4 transcriptional activation domain (AD)-AP-4 ε fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

Insert

AP-4 ε (AP4E1 Human)

Use

Tags

GAL4 transcriptional activation domain (AD) fragm…

Expression

Yeast

Mutation

silent substitutions in codons 905, 1129 and 1137

Promoter

ADH1

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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eSpCas9-EF-5Flnc

Plasmid

#174870

Purpose

CRISPR vector for generating Flnc STREAMING-tag KI cell

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

sgRNA for mouse Flnc (Flnc Synthetic)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-Hs4EHP-S99N_AF

Plasmid

#148692

Purpose

Mammalian Expression of Hs4EHP-S99N

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

Hs4EHP-S99N (EIF4E2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180-E88AN97F_AF

Plasmid

#148688

Purpose

Mammalian Expression of HsGIGYF2_1-180-E88AN97F

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-E88AN97F (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180-LFIENtoDDDAF_AF

Plasmid

#148689

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFIENtoDDDAF

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFIENtoDDDAF (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180-LFIPLtoDDDDA_AF

Plasmid

#148690

Purpose

Mammalian Expression of HsGIGYF2_1-180-LFIPLtoDDDDA

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-LFIPLtoDDDDA (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180_AE

Plasmid

#148578

Purpose

Mammalian Expression of HsGIGYF2_1-180

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180 (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsGIGYF2_1-180-P78DL79A_AE

Plasmid

#148579

Purpose

Mammalian Expression of HsGIGYF2_1-180-P78DL79A

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2017 Jun 1;31(11):1147-1161.

Insert

HsGIGYF2_1-180-P78DL79A (GIGYF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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