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Plasmid#107943PurposeMammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutionsDepositorInserthuman codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
UseTags3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…ExpressionMammalianMutationE174R, S542R, K548R and D908APromoterCAGAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pF1-Hs Cdk1/cyclin B1/Cks1
Plasmid#177329PurposeCo-expression of the human active Cdk1/cyclin B1/Cks1 kinase complexDepositorInsertsUseTags2 x Strep-tag and 8 x HisExpressionInsectMutationPromoterp10 and polhAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-GluA2/GluR2 glutamate receptor [L21/32R-2b]
Plasmid#199405PurposeMammalian expression plasmid of Anti-GluA2/GluR2 glutamate receptor [L21/32R-2b] (Rat). Derived from hybridoma L21/32.DepositorInsertAnti-GluA2/GluR2 glutamate receptor (Rat) recombinant mouse monoclonal antibody (Gria2 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-HA (dark) WPRE
Plasmid#131007PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tagsDepositorInsertsmFP-HA WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsHA - 10 total HA epitope tagesExpressionMammalianMutation"dark" variant with GGG fluorphore comp…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-V5 (dark) WPRE
Plasmid#131006PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tagsDepositorInsertsmFP-V5 WPRE
UseCre/Lox and Synthetic Biology; Mosaic analysis fo…TagsV5 - 10 total of V5 epitope tagExpressionMutation"dark" variant with GGG fluorphore comp…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MADR pDonor smFP-Myc (dark) WPRE
Plasmid#130987PurposeMADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-Myc, a cytoplasmic fluorescent protein which includes multiple Myc epitope tagsDepositorInsertsmFP-Myc WPRE
UseMosaic analysis for dual recombinase-mediated cas…TagsMyc - 10 total Myc tagsExpressionMutation"dark" variant with GGG fluorophore ver…Promoternone (4x polyA to mitigate episomal expression)Available sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-1 in pcDNAI/Amp
Plasmid#54468PurposeAn amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP (1-158)/beta-1 (GNB1 Human, Aequorea victoria)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationGBeta-1 was amplified via PCR, which removed an i…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-Slo1/BKAlpha maxi-K+ channel [L6/60R]
Plasmid#177486PurposeMammalian Expression Plasmid of anti-Slo1/BKAlpha maxi-K+ channel (Mouse). Derived from hybridoma L6/60.DepositorInsertanti-Slo1/BKAlpha maxi-K+ channel (Mus musculus) recombinant mouse monoclonal antibody (Kcnma1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
Anti-GAD65/67 [L127/12R]
Plasmid#177474PurposeMammalian Expression Plasmid of anti-GAD65/67 (Human). Derived from hybridoma L127/12.DepositorUseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
LIR-wtloxP-TRE-CAG-Frt-red-puro-3xPA-Frt-GFP(mVenus)-KrasG12D-cMyc-SV40LT-IRES-KRABoff-PA-TRE-loxP257-RIR
Plasmid#67277Purposeinducbile color change and cancer regulation: FlpO recombinase dependent expression of KrasG12D cMyc and SV40 large T antigen with doxycycline inducible downregulation through tetKRAB system.DepositorInsertsfirst casette contain a red fluorescent protein (katushka) linked to puromycin resitance gene through an E2A site
second cassette contains mVENUS-kras-cmyc-SV40LT-KRABoff
UseTagsmTQ2 blue fluorescent and red flourescent gene ka…ExpressionMammalianMutationPromoterCAGAvailable sinceAug. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-2 in pcDNAI/Amp
Plasmid#54469PurposeAn amino-terminal YFP fragment was fused to Gbeta-2. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-2 (GNB2 Human, Aequorea victoria)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
YFP(1-158)-beta-5 in pcDNAI/Amp
Plasmid#54470PurposeAn amino-terminal YFP fragment was fused to Gbeta-5. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertYFP(1-158)/beta-5 (GNB5 Human, Aequorea victoria)
UseTagsYFP(1-158) was fused to the amino terminus of Gbe…ExpressionMammalianMutationYFP (1-158) includes a substitution of Met for Gl…PromoterAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-SynCAM1 [L45/30R]
Plasmid#177482PurposeMammalian Expression Plasmid of anti-SynCAM1 (Mouse). Derived from hybridoma L45/30.DepositorInsertanti-SynCAM1 (Mus musculus) recombinant mouse monoclonal antibody (Cadm1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238)-beta-1 in pcDNAI/Amp
Plasmid#55592PurposeA carboxyl-terminal CFP fragment was fused to Gbeta-1. When co-expressed with an amino-trerminal CFP or YFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.DepositorInsertCFP (159-238)-Beta 1 (GNB1 Human, Aequorea victoria)
UseTagsCFP(159-238) was fused to the amino terminus of G…ExpressionMammalianMutationCFP(159-238) includes a substitution of His for A…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-dCas9-p300 Core (H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A)
Plasmid#61364Purposeencodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutations H1415A/E1423A/Y1424A/L1428S/Y1430A/H1434A) driven by CMV promoterDepositorInsertS.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9; H1415A/E1423A/Y14…PromoterCMVAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Anti-FGF14/FHF4 [N56/21R-2b]
Plasmid#219479PurposeMammalian Expression Plasmid of anti-FGF14/FHF4 (Mouse) subclass-switched IgG2b R-mAb. Derived from hybridoma N56/21.DepositorInsertAnti-FGF14/FHF4 (Mus musculus) recombinant mouse monoclonal antibody. (Fgf14 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv7.1/KCNQ1 K+ channel [N37A/10R-2b]
Plasmid#220431PurposeMammalian Expression Plasmid of anti-Kv7.1/KCNQ1 K+ channel (Human) subclass-switched IgG2b R-mAb. Derived from hybridoma N37A/10.DepositorInsertAnti-Kv7.1/KCNQ1 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody. (KCNQ1 Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-Kv1.2 K+ channel [K14/16R-rat IgG2a]
Plasmid#222176PurposeMammalian Expression Plasmid of anti-Kv1.2 K+ channel (Human). Derived from hybridoma K14/16.DepositorInsertAnti-Kv1.2 K+ channel (Homo sapiens) recombinant mouse monoclonal antibody. (KCNA2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-CASPR/Neurexin IV [K65/35R-rat IgG2a]
Plasmid#222178PurposeMammalian Expression Plasmid of anti-CASPR/Neurexin IV (Rat). Derived from hybridoma K65/35.DepositorInsertAnti-CASPR/Neurexin IV (Rattus norvegicus) recombinant mouse monoclonal antibody. (Cntnap1 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Anti-GluA2/GluR2 glutamate receptor [L21/32R-rat IgG2a]
Plasmid#222179PurposeMammalian Expression Plasmid of anti-GluA2/GluR2 glutamate receptor (Rat). Derived from hybridoma L21/32.DepositorInsertAnti-GluA2/GluR2 glutamate receptor (Rattus norvegicus) recombinant mouse monoclonal antibody. (Gria2 Chimera: M. musculus (mouse)/R. norvegicus (rat))
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only