Addgene

We narrowed to 11,049 results for: cel.2

Showing: 9681 - 9700 of 11049 results

Results per page:

Sort results by:

Halo-WIPI2B K88E

Plasmid

#175028

Purpose

Expresses WIPI2B (K88E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K88E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 88 to Glutamate (AAG to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-WIPI2B I92E

Plasmid

#175029

Purpose

Expresses WIPI2B (I92E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (I92E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Isoleucine 92 to Glutamate (ATC to GAA)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-WIPI2B C93E

Plasmid

#175030

Purpose

Expresses WIPI2B (C93E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (C93E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Cysteine 93 to Glutamate (TGC to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Halo-WIPI2B K128E

Plasmid

#175031

Purpose

Expresses WIPI2B (K128E) fused to Halo in mammalian cells to abrogate interaction with ATG16L1

Depositor

Andrea Stavoe

Article

Strong et al Elife. 2021 Sep 10;10. pii: 70372. doi:

Insert

WIPI2B (K128E) (WIPI2 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Lysine 128 to Glutamate (AAG to GAG)

Promoter

CMV

Available since

Dec. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAGGS-synPCB2.2

Plasmid

#139479

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-Fnr-Fd-P2A-MTS-HO1-PcyA-FLAG

Use

Tags

Expression

Mammalian

Mutation

V554L

Promoter

CAG promoter

Available since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAM-PAT-ProCDKA;1::CDKA;1:YFP

Plasmid

#80685

Purpose

binary Expression vector for plant expression

Depositor

Nico Dissmeyer, Arp Schnittger

Article

Nowack et al Nature. 2007 May 17;447(7142):312-5. Epu

Insert

CDKA;1 (CDC2 Mustard Weed)

Use

Tags

YFP

Expression

Plant

Mutation

Promoter

ProCDKA;1

Available since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLIB-GST-TEV-RSP3(160-516)

Plasmid

#176692

Purpose

Expression of Chlamydomonas reinhardtii RSP3 recombinant protein in insect cells

Depositor

Ron Vale

Article

Grossman-Haham et al Nat Struct Mol Biol. 2021 Jan;28(1):20-2

Insert

RSP3

Use

Tags

GST

Expression

Insect

Mutation

None

Promoter

polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR-RUNX2(+10A)-IDR-mCherry-CRY2-NLS

Plasmid

#145268

Purpose

Mammalian Expression of Nucelar Opto RUNX2(+10A) IDR with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2(+10A)-IDR (RUNX2 Human)

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST40-CES2C-ΔC-S230A

Plasmid

#200557

Purpose

Mammalian expression of secreted mouse CES2C S230A mutant

Depositor

Jonathan Long

Article

Wei et al Cell Metab. 2023 Apr 28:S1550-4131(23)00

Insert

Mouse Carboxylesterase 2C S230A mutant delta C-terminal (Ces2c Mouse)

Use

Tags

6xHis, Flag, and V5

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1-LanCL2-C321A/C367A-C-Ter-His

Plasmid

#154188

Purpose

To express LanCL2-C321A/C367A in bacterial cells

Depositor

Wilfred van der Donk

Article

Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

Insert

LanCL2

Use

Tags

Hexahistidine

Expression

Bacterial

Mutation

Cys321->Ala321; Cys367->Ala367

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(WT)-mouse-full-length-CHMP3-HA

Plasmid

#154175

Purpose

expresses mouse CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α-mouse-CHMP3(147)-HA

Plasmid

#154177

Purpose

expresses mouse CHMP3(147) in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3 (Chmp3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

C-terminal truncation of CHMP3 at amino acid 147

Promoter

EF1alpha

Available since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-mRIN2 dRA/PINCO

Plasmid

#108938

Purpose

Retroviral expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

LTR

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HA-mRIN2 dRHVPS9/PINCO

Plasmid

#108939

Purpose

Retroviral expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

LTR

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIR-Report-SPRED1-3'UTR (containing miR-21 sites)

Plasmid

#62579

Purpose

Translational Luciferase Reporter encoding a fragment of the 3'UTR of SPRED1 containing two miR-21 binding sites (sites 1 and 2)

Depositor

Michael Ruppert

Article

Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6

Insert

SPRED1 (SPRED1 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only
pFUW-tetO-3xFLAG-GATA2

Plasmid

#125600

Purpose

doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in mammalian cells

Depositor

Filipe Pereira

Article

Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

Insert

GATA binding protein 2 (GATA2 Human)

Use

Lentiviral

Tags

3xFLAG tag

Expression

Mammalian

Mutation

Promoter

TRE-mCMV

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFRT-TO-RPB1-His WT CRres si2,4R

Plasmid

#139404

Purpose

Dox-inducible, wt C-terminal His tagged RPB1, resistant to siRNAs 2 & 4, for generation of TRex flp In cells.

Depositor

Jesper Svejstrup

Article

Tufegdzic Vidakovic et al Cell. 2020 Mar 5. pii: S0092-8674(20)301

Insert

RPB1 (POLR2A Human)

Use

Tags

6xHis

Expression

Mammalian

Mutation

Promoter

pCMV, TRex

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Gfp-zf-vasa

Plasmid

#167323

Purpose

Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of zebrafish by in vitro transcription.

Depositor

Goro Yoshizaki

Article

Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

Insert

zf vasa 3'UTRs (ddx4 Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
WT1 KI gRNA1

Plasmid

#92312

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Insert

WT1 (WT1 Human)

Use

Tags

CRISPR GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
WT1 KI gRNA2

Plasmid

#92313

Purpose

CRISPR-GFP-gRNA for cutting WT1

Depositor

Sean Palecek

Article

Bao et al Nat Biomed Eng. 2016;1. pii: 0003. doi:

Insert

WT1 (WT1 Human)

Use

Tags

CRISPR GFP

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge