-
Plasmid#210270PurposeN-terminal EGFP-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.DepositorTypeEmpty backboneUseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only
-
pKB33 (pUC19-KANMX-tetO7-CYCpr)
Plasmid#210472PurposePlasmid with KanMX-tetO7-CYCpr cassette from the yTHC collection. Used for amplifying cassette with homology to regions of interest for genomic integration.DepositorInsertKanMX-TetO7-CYCpr cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
(F) pXYL (G) P1 (SBE216)
Plasmid#195017Purposepromoter of the gene xylose reductase from R. toruloides for the position P2 (F/G)DepositorInsert(F) pXYL (G)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R) pXYL (D) P1 (SBE215)
Plasmid#195016Purposepromoter of the gene xylose reductase from R. toruloides for the position P1 (R/D)DepositorInsert(R) pXYL (D)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(R)pADH2(D)_P1(SBE214)
Plasmid#195015Purposepromoter of the gene alcohol dehydrogenase 2 from R. toruloides for the position P1DepositorInsert(B) pADH2 (R)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(J) crtYB(K) G3 (SBE117)
Plasmid#194996PurposeRhodotorula toruloides gene phytoene synthase/lycopene cyclase (crtYB, RHTO_04605) for G3DepositorInsert(J) crtYB(K)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(G) crtI (H) G2 (SBE116)
Plasmid#194995PurposeRhodotorula toruloides gene phytoene dehydrogenase (crtI, RHTO_04602) for G2DepositorInsert(G) crtI (H)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(D) crtE (E)_G1 (SBE115)
Plasmid#194994PurposeRhodotorula toruloides gene geranylgeranyl diphosphate synthase (crtE, RHTO_02504) for G1DepositorInsert(D) crtE (E)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(I) pGPD (J)_P3 (SBE111)
Plasmid#194990Purposepromoter of the gene glyceraldehyde-3-phosphate dehydrogenase from R. toruloides for a P3 position (I/J)DepositorInsert(I) pGPD (J)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(S) insD_ku70 (Q) (SBE139)
Plasmid#187627Purposethe insertional region downstream for the deletion of the KU70 gene in R. toruloides. Overhangs S/QDepositorInsert(S) insD KU70 (Q)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
(P) insUP_ku70 (SBE138) (B)
Plasmid#187626Purposethe insertional region upstream for the deletion of the KU70 gene in R. toruloides. Overhangs P/BDepositorInsert(P) insUP KU70 (B)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-dMASS_1_WPRE
Plasmid#209765PurposeAAV virus production for neuronal expression of dMASS_1 under hSyn promoterDepositorInsertdMASS_1
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
M_NES 54kDa
Plasmid#201375PurposeNucleocytoplasmic transport probeDepositorInsertMAPK_NES-EGFP-4PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10D(E215Q)-HA
Plasmid#209248PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseTagsHAExpressionMammalianMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10D(E215Q)-HA
Plasmid#209260PurposeMammalian expression of ATP10D mutant E215QDepositorInsertATP10D (ATP10D Human)
UseRetroviralTagsHAExpressionMutationE215QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG/ATP10B(E210Q)-HA
Plasmid#209247PurposeMammalian expression of ATP10B mutant E210QDepositorInsertATP10B (ATP10B Human)
UseTagsHAExpressionMammalianMutationE210QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B-HA
Plasmid#209233PurposeMammalian expression of ATP10BDepositorInsertATP10B (ATP10B Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10B(E210Q)-HA
Plasmid#209259PurposeMammalian expression of ATP10B mutant E210QDepositorInsertATP10B (ATP10B Human)
UseRetroviralTagsHAExpressionMutationE210QPromoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMx/ATP10A-HA
Plasmid#209232PurposeMammalian expression of ATP10ADepositorInsertATP10A (ATP10A Human)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceNov. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQE60 R196M wgMDH
Plasmid#204273PurposeBacterial expression of R196M wgMDH with IPTG induction - Active site mutantInsertMDHG_CITLA
UseTags6X HisExpressionBacterialMutationR196MPromoterAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only