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Plasmid#207461PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertTdTc302 (Dntt Mouse)
UseTags10xHisExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
p Pcrg1: Khd4-Ada-Gfp (CbxR)
Plasmid#203734PurposePlasmid for ectopic integration and expression of Khd4-Ada-Gfp. The 4282 bp khd4-ORF is fused C-terminally to the 1200 bp adar catalytic domain (Ada) with three repeats of GGGGS linker in between, folDepositorInsertKhd4-Ada-Gfp
UseTagsGfpExpressionBacterialMutationPromoterPcrg1Available sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNMSB96
Plasmid#199316PurposeMosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605DepositorInsertflp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
UseTagsExpressionWormMutationmTagBFP2 from pJJR81, C. elegans codon optimized …Promoterflp-18Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 - circMAN2A1
Plasmid#198837PurposeThis contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon twoDepositorInsertcircular mannosidase alpha class 2A member 1
UseTagsExpressionMammalianMutationcircular RNA composed by 2 exonsPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-Duet-6xHis-TEV-Nsp8(76-198)
Plasmid#201024PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 residues 76-198, with an N-terminus His tag followed by a TEV cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
UseTags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLX317-VRK2
Plasmid#199641PurposeExpresses VRK2DepositorInsertVRK2 (VRK2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-ATG13(Δ1-198)
Plasmid#186222PurposeFor expression of ATG13(Δ1-198) tagged with HADepositorInsertATG13 (ATG13 Human)
UseRetroviralTagsHAExpressionMammalianMutationDeleted amino acids 1 - 198PromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-GST-TEV-NAP1 (WT)
Plasmid#198034PurposeUsed for the expression and purification of NAP1 in a mammalian expression system (SMC Internal No. 2064)DepositorInsertNAP1 (AZI2 Human)
UseTagsGST-TEVExpressionMammalianMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)
Plasmid#198072PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationTBK1ΔCTD (res 1-666)PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TEV-mCherry-TBK1
Plasmid#198033PurposeUsed for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV and mCherryExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shPP1C #2
Plasmid#198763Purposeconditional knockdown of PP1CDepositorInsertshPP1C #2 (PPP1CC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-Teton-puro-shPP1C #1
Plasmid#198762Purposeconditional knockdown of PP1CDepositorInsertshPP1C #1 (PPP1CC Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Sniper2P
Plasmid#193857PurposeExpresses human codon-optimized Sniper2P and blasticidin resistance: EFS promoter-Sniper2P-NLS-FLAG-P2A-BSDDepositorInsertSniper2L
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationE1007LPromoterEFSAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GGSG_M
Plasmid#146950PurposeInsect Expression of DmAGO1-del942-971-GGSGDepositorInsertDmAGO1-del942-971-GGSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-del942-971-GSG_M
Plasmid#146951PurposeInsect Expression of DmAGO1-del942-971-GSGDepositorInsertDmAGO1-del942-971-GSG (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798AQ800A_M
Plasmid#146952PurposeInsect Expression of DmAGO1-E798AQ800ADepositorInsertDmAGO1-E798AQ800A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-E798QQ800E_M
Plasmid#146953PurposeInsect Expression of DmAGO1-E798QQ800EDepositorInsertDmAGO1-E798QQ800E (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941A_M
Plasmid#146954PurposeInsect Expression of DmAGO1-H941ADepositorInsertDmAGO1-H941A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-H941K_M
Plasmid#146955PurposeInsect Expression of DmAGO1-H941KDepositorInsertDmAGO1-H941K (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only