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Plasmid#62935PurposeExpression of dCas9-VP64-6xHis in bacterial cellsDepositorInsertdCas9-VP64
UseTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A and H840A amino acid changes render Cas9 nuc…PromoterT7Available sinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1 HA-FAM208A
Plasmid#115845PurposeEncodes codon optimized HA tagged FAM208ADepositorInsertHA tagged FAM208A (C3orf63 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_nV5-AMOTL2
Plasmid#172999Purposeconstitutive expression of V5-tagged AMOTL2DepositorInsertV5-AMOTL2 (AMOTL2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-eNme2-C-ABE8e
Plasmid#185667PurposeExpresses eNme2-C-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-neNme2-C
UseCRISPRTagsExpressionMammalianMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…PromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE-3
Plasmid#72678PurposeExpresses Lambda Red recombinases and a dominant negative MutL allele all controlled by temperature sensitve cI857 repressor for high precision and efficiency MAGE experiments. Kan resistance marker.DepositorInsertmutL E32K
UseTagsnoneExpressionBacterialMutationE32K mutation conferring dominant mutator phenoty…PromoterAvailable sinceFeb. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmaxRNaseHtrunc
Plasmid#207858PurposeMammalian expression of PEmaxDRNaseH prime editorDepositorInsertPEmaxDRNaseH
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrtPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 barr1 flag
Plasmid#14687DepositorInsertbeta arrestin 1 (Arrb1 Rat)
UseTagsflagExpressionMammalianMutationPromoterAvailable sinceJune 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-hAtg13
Plasmid#22875DepositorInsertautophagy-related genes 13 (ATG13 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2010AvailabilityAcademic Institutions and Nonprofits only -
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CMV-Nme2ABE8e
Plasmid#189928PurposePlasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.DepositorInsertNme2ABE8e
UseTagsNLSExpressionMammalianMutationNme2Cas9 D16APromoterCMVAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 p18 shRNA
Plasmid#26631DepositorInsertp18 shRNA (LAMTOR1 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-mSin1(1.1)-HA
Plasmid#73388PurposeExpresses mSin-HADepositorInsertmSin1 transcript variant protein isoform 1 (MAPKAP1 Human)
UseTagsHA tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 MP1 shRNA
Plasmid#26632DepositorInsertMP1 shRNA (LAMTOR3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceNov. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (71-181)
Plasmid#86753PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 71-181PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (1-181)
Plasmid#86752PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertatg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 1-181PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Atg14 (1-200)
Plasmid#86750PurposeN-terminal myc-tagged protein expression in mammalian cellsDepositorInsertAtg14 (ATG14 Human)
UseTagsmycExpressionMammalianMutationaa 1-200PromoterCMVAvailable sinceJan. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Pansio
Plasmid#214445PurposeExpresses eSpCas9 and gRNA targeting Pansio-1 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA3-Olônne
Plasmid#214447PurposeExpresses eSpCas9 and gRNA targeting Olônne-18 GSHDepositorInserteSpCas9-P2A-mRuby2-P2A-tp53dn
UseCRISPRTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIA10.53-Clover
Plasmid#214450PurposeBxbI landing-pad mClover-IRES-puro donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates constitutively expressing cell line.DepositorInsertmClover
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only