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YIp211-CUP1-NUbo-E3(63) I227A-VSV

Plasmid

#212757

Purpose

Copper-inducible expression of NUbo-E3(63) I227A-VSV in budding yeast, reduced activity of E3(63)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(63) I227A

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Pib1(I227A)

Promoter

CUP1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212740

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-mCherry-VSV

Plasmid

#212739

Purpose

Consitutive expression of myc-E3(1)-CUbo-mCherry-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-mCherry-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-ADH-myc-E3(1)-CUbo-NLS-VSV

Plasmid

#212737

Purpose

Consitutive expression of myc-E3(1)-CUbo-NLS-VSV in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGMC00033 (aka pMC0224)

Plasmid

#210750

Purpose

Guide RNA targeting SERPINB3

Depositor

Raj Chari

Article

Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

Insert

SERBPINB3 guide RNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(13))-PGKpuro2ABFP-W

Plasmid

#208552

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(13) (RUNX3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RUNX3(12))-PGKpuro2ABFP-W

Plasmid

#208551

Purpose

Lentiviral vector expressing gRNA targeting human RUNX3

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RUNX3(12) (RUNX3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(AAVS1)-PGKpuro2ABFP-W

Plasmid

#200459

Purpose

Lentiviral vector expressing gRNA targeting human AAVS1

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

AAVS1 (AAVS1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(1))-PGKpuro2AmCherry-W

Plasmid

#210612

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(1)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Plasmid

#212805

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(16))-PGKpuro2ABFP-W

Plasmid

#200491

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(16) (RIC8A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(RIC8A(15))-PGKpuro2ABFP-W

Plasmid

#200490

Purpose

Lentiviral vector expressing gRNA targeting human RIC8A

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

RIC8A(15) (RIC8A Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

Plasmid

#210613

Purpose

Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Insert

Control(2)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 8, 2024

Availability

Academic Institutions and Nonprofits only

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Lnig-INTR-GFP

Plasmid

#208904

Purpose

encodes the inotocin receptor (oxytocin/vasopressin-like) from the ant Lasius niger, tagged with GFP

Depositor

Christian Gruber

Article

Di Giglio et al Sci Rep. 2017 Feb 1;7:41002. doi: 10.103

Insert

INTR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTdelta5cys

Plasmid

#207465

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) surface-cysteine free variant

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTdelta5cys (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Cys188Ala, Cys216Ser, Cys302Ala, Cys378Ala, and C…

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTc253

Plasmid

#207464

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 253

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

MTdTc253 (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Ser253Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTc188

Plasmid

#207463

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 188

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTc188 (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Cys216Ser, Cys302Ala, Cys378Ala, and Cys438Ser

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pET19-TdTc180

Plasmid

#207462

Purpose

Bacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 180

Depositor

Jay Keasling

Article

Palluk et al Nat Biotechnol. 2018 Aug;36(7):645-650.

Insert

TdTc180 (Dntt Mouse)

Use

Tags

10xHis

Expression

Bacterial

Mutation

Glu180Cys, Cys188Ala, Cys216Ser, Cys302Ala, Cys37…

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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