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Showing: 9601 - 9620 of 51990 results

  1. pET15-EF1n3_mFAP2b

    Plasmid
    #159472
    Purpose
    Encodes for EF1n3_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1n3_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    159472-plasmid-map-sequence-id-319720

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  2. pET15-EF1n_mFAP2b

    Plasmid
    #159473
    Purpose
    Encodes for EF1n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF1n_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    159473-plasmid-map-sequence-id-316660

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  3. pAcBac1-haloTyrRS C6

    Plasmid
    #141174
    Purpose
    Expression of orthogonal tRNA and tRNA synthetase for incorporation of unnatural amino acids
    Depositor
    Ryan Mehl
    Insert
    C6 HaloTyr tRNA synthetase
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    L270S, Y271L, N311G, C313T
    Promoter
    CMV
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    141174-plasmid-map-sequence-id-314452

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  4. pET15-EF2n_mFAP2b

    Plasmid
    #159468
    Purpose
    Encodes for EF2n_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159468-plasmid-map-sequence-id-316657

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  5. pET15-EF2n2_mFAP2b

    Plasmid
    #159467
    Purpose
    Encodes for EF2n2_mFAP2b with an N-terminal 6xHis tag, thrombin epitope, and TEV epitope. Expression is controlled by the T7 promoter with E. coli codon optimization.
    Depositor
    David Baker
    Insert
    EF2n2_mFAP2b
    Use
    Tags
    6xHis-thrombin-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    159467-plasmid-map-sequence-id-316656

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  6. pZNF624-donor

    Plasmid
    #132511
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF624 (ZNF624 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    none
    Promoter
    Available since
    March 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    132511-plasmid-map-sequence-id-275217

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  7. pENTR-R2L3-cGR-IRESpuro-pA

    Plasmid
    #139538
    Purpose
    Provides GR protein tag N-terminal to IRES-puro-pA cassette on Gateway entry vector
    Depositor
    Minoru Ko
    Insert
    GR
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139538-plasmid-map-sequence-id-274620

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  8. pZNF8-donor

    Plasmid
    #136689
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF8 (ZNF8 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    136689-plasmid-map-sequence-id-274397

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  9. U24m

    Plasmid
    #121026
    Purpose
    MoClo golden gate assembly BC part for RiboJ-BCD2 (low expression bi-cistronic RBS, engineered for context-independence, prefixed by self-cleaving RiboJ; see https://doi.org/10.1038/nmeth.2404).
    Depositor
    Richard Murray
    Insert
    RiboJ + BCD2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121026-plasmid-map-sequence-id-238071

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  10. T14m

    Plasmid
    #121033
    Purpose
    MoClo golden gate assembly DE part for L3S1P13 (178x-strength synthetic terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    L3S1P13 (synthetic Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121033-plasmid-map-sequence-id-236356

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  11. C90m

    Plasmid
    #120998
    Purpose
    MoClo golden gate assembly CD part for NahR-sfGFP fusion (Adam Meyer improved NahR (C75m) fused to superfolder GFP (C3m)). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    NahRsfGFP fusion
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120998-plasmid-map-sequence-id-236128

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  12. C92m

    Plasmid
    #121000
    Purpose
    MoClo golden gate assembly CD part for sfCFP-pdt3 (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with a pdt3 tag for mfLon protease).
    Depositor
    Richard Murray
    Insert
    sfCFPpdt3 (Chen,Bennett)
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121000-plasmid-map-sequence-id-238058

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  13. C99m

    Plasmid
    #121003
    Purpose
    MoClo golden gate assembly CD part for codon-optimized mScarlet-I (fast-folding mScarlet variant, codon-optimized for E. coli; see https://www.fpbase.org/protein/mscarlet-i/).
    Depositor
    Richard Murray
    Insert
    mScarlet-I codon optimized
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121003-plasmid-map-sequence-id-236141

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  14. C100m

    Plasmid
    #121004
    Purpose
    MoClo golden gate assembly CD part sfYFP-ptd3 (superfolder YFP with pdt3 degradation tag for mfLon). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121004-plasmid-map-sequence-id-236143

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  15. C101m

    Plasmid
    #121005
    Purpose
    MoClo golden gate assembly CD part sfCFP-LAA (superfolder CFP developed by Ye Chen in Matthew Bennet's lab, with LAA degradation tag for ssrA).
    Depositor
    Richard Murray
    Insert
    sfCFP with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121005-plasmid-map-sequence-id-236158

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  16. C102m

    Plasmid
    #121006
    Purpose
    MoClo golden gate assembly CD part treRT-LAA (synthetic Lac-family repressor chimera with LAA degradation tag for ssrA; see DOI: 10.1021/sb500262f).
    Depositor
    Richard Murray
    Insert
    treRT with LAA tag
    Use
    Synthetic Biology
    Tags
    LAA degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121006-plasmid-map-sequence-id-236159

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  17. C72m

    Plasmid
    #120984
    Purpose
    MoClo golden gate assembly CD part for LacI-MVY (Adam Meyer's improved LacI repressor from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    LacI Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120984-plasmid-map-sequence-id-236046

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  18. C73m

    Plasmid
    #120985
    Purpose
    MoClo golden gate assembly CD part for LasR-LSTPLVMI (Adam Meyer's improved LasR activator, selected for dynamic range and low cross-sensitivity with methods from https://doi.org/10.1101/285866).
    Depositor
    Richard Murray
    Insert
    LasR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120985-plasmid-map-sequence-id-236047

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  19. C74m

    Plasmid
    #120986
    Purpose
    MoClo golden gate assembly CD part for LuxRQ-FMY (Adam Meyer's improved LuxR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    LuxR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120986-plasmid-map-sequence-id-239311

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  20. C75m

    Plasmid
    #120987
    Purpose
    MoClo golden gate assembly CD part for NahRQ-VHAC (Adam Meyer's improved NahR activator from https://doi.org/10.1101/285866, selected for dynamic range and low cross-sensitivity).
    Depositor
    Richard Murray
    Insert
    NahR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    120987-plasmid-map-sequence-id-236049

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Showing: 9601 - 9620 of 51990 results