Addgene

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pWZL Neo Myr Flag ADCK5

Plasmid

#20416

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ADCK5 (ADCK5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag HIPK1

Plasmid

#20498

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

HIPK1 (HIPK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K6

Plasmid

#20519

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K6 (MAP3K6 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pExp-His-zBasic-RBD

Plasmid

#194999

Purpose

Production of SARS-CoV2 receptor binding domain in E. coli

Depositor

Marko Hyvönen

Article

Gaynor et al Nat Commun. 2023 Jun 16;14(1):3583. doi:

Insert

RBD (S SARS-CoV-2)

Use

Tags

His8-Zbasic

Expression

Bacterial

Mutation

Promoter

T7lac

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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MBP-g0s2

Plasmid

#179294

Purpose

E. coli expression plasmid (Ptac promoter) for full-length human G0S2 with N-terminal mature maltose binding protein + enterokinase cleavage site

Depositor

Debra Hansen

Article

Moran et al PLoS One. 2021 Jul 14;16(7):e0249164. do

Insert

G0S2 (G0S2 Human)

Use

Tags

mature maltose binding protein + enterokinase cle…

Expression

Bacterial

Mutation

full-length G0S2, residues 1-103 from accession n…

Promoter

Ptac

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CDK4

Plasmid

#20448

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CDK4 (CDK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_IKBKG_WT

Plasmid

#82242

Purpose

Gateway Donor vector containing IKBKG , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

IKBKG (IKBKG Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_FBXW7_p.R658Q

Plasmid

#81502

Purpose

Gateway Donor vector containing FBXW7 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

FBXW7 (FBXW7 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

R658Q

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CAMK4

Plasmid

#20441

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMK4 (CAMK4 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAPKAP1

Plasmid

#20528

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPKAP1 (MAPKAP1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K8

Plasmid

#20521

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K8 (MAP3K8 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_ERBB2_p.V842I

Plasmid

#81722

Purpose

Gateway Donor vector containing ERBB2, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ERBB2 (ERBB2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

I655V; V842I

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CLK3

Plasmid

#20465

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CLK3 (CLK3 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag CALM2

Plasmid

#20435

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CALM2 (CALM2 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pDONR223_MAP2K1_p.E203K

Plasmid

#81729

Purpose

Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, David Root, William Hahn

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

MAP2K1 (MAP2K1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

L197P; E203K; I310N

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag IKBKE

Plasmid

#20504

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

IKBKE (IKBKE Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag CMPK

Plasmid

#20537

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CMPK (CMPK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag ITPK1

Plasmid

#20508

Depositor

Jean Zhao, William Hahn

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

ITPK1 (ITPK1 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pXPR_BRD003-sgWRN-EIJ

Plasmid

#125774

Purpose

constitutive expression of a guide RNA targeting an exon-intron junction of human WRN

Depositor

Francisca Vazquez

Article

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

sgWRN-EIJ (WRN Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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