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Plasmid#61790PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutatedDepositorInsertmouse Map3k1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseTagsExpressionMutationboth miR-200c MRE-2 and MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT
Plasmid#61786PurposeLuciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutatedDepositorInsertmouse Crtap 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLexA::SRDX (GB0732)
Plasmid#68184PurposeProvides the LexA Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertLexA Binding Domain fused to SRDX Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSR11
Plasmid#69154Purposeread-outloxP mCherry to GFP switch, with fzr-1 promoter, gene and UTR, for integration on cxtTi10816, Mos Chr IVDepositorInsertsUseCre/Lox; MossciTagsExpressionWormMutationcodon-optimzed index 1.0Promoterfzr-1 and rps-27Available sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pES006 (pTet-qacR 2/pQacA-deGFP)
Plasmid#69035Purposereporter with qacR 2 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationE57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…PromoterpQacA and pTetAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGal4BD::BDR (GB0734)
Plasmid#68185PurposeProvides the Gal4 Binding Domain fused to the BDR Transcriptional Repressor as a level 0 GoldenBraid partDepositorInsertGal4 Binding Domain fused to BDR Transcriptional Repressor
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL836
Plasmid#62827PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::Asp terminator + PLtetO-1::RBS (st7) hfq::T1 terminator + ColE1DepositorInsertsgfp
ipex leader
hfq leader
UseSynthetic BiologyTagsompC leader and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2107
Plasmid#62841PurposeLim Lab plasmid: AmpR + PconNoHindM8::micC::Asp terminator + PLlacO-1::T710::hfq::T1 terminator + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsm8::micC leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialMutationPromoterpConNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238) in pcDNAI/Amp
Plasmid#60896PurposeThis vector attaches CFP(159-238) to the N-terminus of a protein.DepositorInsertCFP(159-238)
UseTagsExpressionMammalianMutationAsn-164 to HisPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Fhod1-3'UTR-MRE-2 MUT
Plasmid#61787PurposeLuciferase reporter containing the 3'UTR of mouse Fhod1 with MRE-2 mutatedDepositorInsertmouse Fhod1 3'UTR with miR-200c MRE-2 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-2 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Tob1-3'UTR-MRE-1 MUT
Plasmid#61788PurposeLuciferase reporter containing the 3'UTR of mouse Tob1 with MRE-1 mutatedDepositorInsertmouse Tob1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Zfp36-3'UTR-MRE-1 MUT
Plasmid#61794PurposeLuciferase reporter containing the 3'UTR of mouse Zfp36 with MRE-1 mutatedDepositorInsertmouse Zfp36 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Snail1-3'UTR-MRE-1 MUT
Plasmid#61795PurposeLuciferase reporter containing the 3'UTR of mouse Snail1 with MRE-1 mutatedDepositorInsertmouse Snail1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Mapk12-3'UTR-MRE-1 MUT
Plasmid#61797PurposeLuciferase reporter containing the 3'UTR of mouse Mapk12 with MRE-1 mutatedDepositorInsertmouse Mapk12 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO myc Separase delta 55
Plasmid#59824PurposeAllows the integration of myc Separase delta 55 in the genome and Tet-inducible expression.DepositorInsertSeparase (ESPL1 Human)
UseTagsMycExpressionMammalianMutationdelta 55Promoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-hRIP1-FL I541P
Plasmid#61381PurposeExpresses Human full length RIPK1 with I541P mutation in the mammalian cellsDepositorInsertFull length human RIP1with I541P mutation (RIPK1 Human)
UseTagsEGFP and HA tagExpressionMammalianMutationchanged I541 to PPromoterAvailable sinceJan. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
mKO-Alpha-Actinin-19
Plasmid#57831DepositorInsertAlpha-Actinin (ACTN1 Human)
UseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcdna3 T7 Jip1b(C631S, C643S and C699T)
Plasmid#52128Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(M1-5) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationC631S, C643S and C699TPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 T7 Jip1b(471-707)
Plasmid#52428Purposea mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.DepositorInsertJNK interacting protein-1b (JIP-1b)(471-707) ) (Mapk8ip1 Mouse)
UseTagsT7ExpressionMammalianMutationdeleted amino acid 1-470PromoterAvailable sinceApril 29, 2014AvailabilityAcademic Institutions and Nonprofits only