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pET28a-AzaD

Plasmid

#224425

Purpose

Express AzaD in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaD

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaB

Plasmid

#224423

Purpose

Express AzaB in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaB

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaG

Plasmid

#224427

Purpose

Express AzaG in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaG

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-AzaE

Plasmid

#224426

Purpose

Express AzaE in E. coli

Depositor

Emily Balskus

Article

Van Cura et al Angew Chem Int Ed Engl. 2023 Jul 10;62(2

Insert

azaE

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq RNA Polymerase

Plasmid

#215349

Purpose

Express T. aq RNA Polymerase subunits in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

RNA polymerase

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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T. aq sigmaA

Plasmid

#215350

Purpose

Express T. aq sigmaA transcription initiation factor in E. coli cells

Depositor

Julius Lucks

Article

Hertz et al Nucleic Acids Res. 2024 May 8;52(8):4466

Insert

rpoD

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Use

Tags

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP

Plasmid

#184819

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Backbone into which homology arms are cloned for CRISPR/Cas9-mediated integration of QF2.

Depositor

Marnie Halpern

Article

Spikol et al eLife 12:RP89516

Insert

QF2-pA-he1.1:YFP

Use

CRISPR; Backbone for cloning of homology arms (fo…

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pPRISM-QF2-he1.1:YFP-rln3a-HA

Plasmid

#184820

Purpose

Modified version of GeneWeld method from Wierson et al., 2020 (https://doi.org/10.7554/eLife.53968). Contains homology arm inserts for integration of QF2 into rln3a gene by CRISPR/Cas9 integration.

Depositor

Marnie Halpern

Article

Spikol et al eLife 12:RP89516

Insert

rln3a-HA

Use

CRISPR; For integration of qf2 by homology-direct…

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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TOPO-nmba

Plasmid

#184821

Purpose

Used to synthesize RNA probe for in situ hybridization for nmba

Depositor

Marnie Halpern

Article

Spikol et al eLife 12:RP89516

Insert

nmba (nmba Zebrafish)

Use

Template for synthesis of nmba probe

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-Rta 125-175

Plasmid

#99668

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (125-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-VP64-RTA(75-190)

Plasmid

#99669

Purpose

Expresses dSp Cas9 fused to combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

VP64-RTA(75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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dSp-p65 (1-100)

Plasmid

#99660

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (1-100)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (50-150)

Plasmid

#99661

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (50-150)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta Full (1-190)

Plasmid

#99662

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (1-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-190

Plasmid

#99663

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-190)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-Rta 75-175

Plasmid

#99666

Purpose

Expresses dSp Cas9 fused to truncated RTA activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated RTA activation domain (75-175)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (150-261)

Plasmid

#99655

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (150-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (100-261)

Plasmid

#99656

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (100-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
dSp-p65 (200-261)

Plasmid

#99657

Purpose

Expresses dSp Cas9 fused to truncated p65 activation domain

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

Tags

truncated p65 activation domain (200-261)

Expression

Mammalian

Mutation

dead Cas9

Promoter

Available since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge