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Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9-KRAB
Plasmid#110822PurposePiggyBac compatible plasmid expressing dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-dSaCas9-NLS-VPR
Plasmid#68495PurposeAAV vector containing nuclease null SaCas9 fused to VPRDepositorInsertdSaCas9
UseAAVTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynap-FLEX.SF-iGluSnFR.S72A
Plasmid#106182PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVTagsExpressionMutationGltI: S72APromoterhSynapsinAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWG-dCas9-VPR
Plasmid#63802PurposeSP-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; drosophila vectorDepositorInsertSP-dCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionInsectMutationPromoteract5cAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit g2SE
Plasmid#49170PurposepHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSynapsin.SF-iGluSnFR.A184V
Plasmid#106175PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterhSynapsinAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GST scFv [N100/13]
Plasmid#190521PurposeMammalian Expression of GST scFV. Derived from hybridoma N100/13.DepositorInsertGST (Schistosoma japonicum) recombinant scFV
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/38]
Plasmid#182095PurposeMammalian Expression of GFP scFv. Derived from hybridoma N86/38.DepositorInsertrecombinant mouse scFv targeting GFP
UseTagsHA, HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5-LAP-MPS1-WT
Plasmid#114044PurposeExpression of exogenous LAP tagged MPS1 WTDepositorInsertMPS1-WT (TTK Human)
UseTagsExpressionMammalianMutationWT MPS1 sequencePromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/20]
Plasmid#206744PurposeMammalian Expression of GFP scFV. Derived from hybridoma N86/20 scFv.DepositorInsertGFP (Aequorea victoria) recombinant scFV
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmiRA1-MYB
Plasmid#141120PurposeLentiviral overexpression of MYB in mammalian cells.DepositorInsertMYB (MYB Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-CAGGS-dCas9
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
GABA(A) receptor subunit B3SE
Plasmid#49171PurposepHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surfaceDepositorInsertGABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
UseTagsmyc, pHGFP (pHluorin), and thrombin cleavage site…ExpressionMammalianMutationGTG (V) to TGC (C)PromoterCMVAvailable sinceDec. 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184S
Plasmid#106198PurposeTight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184S
UseAAVTagsExpressionMutationGltI: A184SPromoterCAGAvailable sinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP scFv [N86/8]
Plasmid#199429PurposeMammalian expression of GFP scFv. Derived from hybridoma N86/8.DepositorInsertrecombinant mouse scFv targetingGFP (Aequorea victoria)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV_NLS-SaCas9-NLS-VPR
Plasmid#68496PurposeAAV vector containing SaCas9 fused to VPRDepositorInsertSaCas9-VPR
UseAAV and CRISPRTagsVPRExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pF712-pET28a-Hs-delta44-GCDH-NHis wt
Plasmid#85113Purposeexpression of recombinant human mature (delta44) GCDH wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta44) GCDH (GCDH Human)
UseTags6xHisExpressionBacterialMutationsilent SNP: G1041TPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.SF-iGluSnFR.A184V
Plasmid#106199PurposeMedium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.A184V
UseAAVTagsExpressionMutationGltI: A184VPromoterCAGAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only