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Plasmid#59701Purposeto make Cre-ERT2 expressing stable cell line by retrovirusDepositorInsertCre-ERT2
UseRetroviralTagsEstrogen receptor (ERT)ExpressionMammalianMutationPromotercmvIEAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGMC00009 (aka pRC0494)
Plasmid#195294PurposeLenti-CRISPR-V2-GFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/GFPDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro PTEN shRNA
Plasmid#10669DepositorInsertPTEN shRNA (PTEN Human)
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
CROPSeq-PP7-p65-HSF
Plasmid#196719PurposeCROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).DepositorTypeEmpty backboneUseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)
Plasmid#215010PurposeExpress a Mitophagy reporter in mammalian cells (untested)DepositorInsertpSu9 (Presequence of Fo-ATPase subunit 9)
UseLentiviralTagsHaloTag(7)-mGFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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mem-mCherry-pTRIP
Plasmid#163521PurposeUsed to construct lentivirus to express membrane-targeted mCherry in mammalian cellsDepositorInsertmembrane targeted mCherry
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBabepuro-PDGFB
Plasmid#189611PurposeRetroviral expression vector for PDGFB. stimulates fibroblastic proliferation (Tchenio T, unpublished, 2008)DepositorInsertPDGFB (PDGFB Human)
UseRetroviralTagsExpressionMutationPromoterSV40 early promoterAvailable sinceAug. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ (M)-YFP-Eed
Plasmid#82512PurposeLentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.DepositorInsertembryonic ectoderm development
UseLentiviralTagsHaloTagExpressionMammalianMutationinsert contains AA39-441 of EeDPromotercmvAvailable sinceDec. 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGMC00010 (aka pRC0495)
Plasmid#195295PurposeLenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFPDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-hPPM1B
Plasmid#185383PurposeFor mammalian expression of shRNA: GAGCAGAAGAGGATGAATTTA that targets human PPM1BDepositorInsertPPM1B (PPM1B Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
TLCV2 sgAAVS1
Plasmid#127099PurposeEncodes gRNA for human AAVS1.DepositorInsertAAVS1 (PPP1R12C AAVS1 Human) (PPP1R12C Human)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
1039 pBabe puroL PTEN A4
Plasmid#10941DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutationA4: S380A, T382A, T383A, S385APromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
Clim-DC1-mCherry-H2B-Venus
Plasmid#129559Purposenuclear markerDepositorInsertmcherry and Histone 2B fused to venus
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
1038 pBabe puroL PTEN 360-364A
Plasmid#10940DepositorInsertPTEN (PTEN Human)
UseRetroviralTagsExpressionMammalianMutation360-364A (SSSTS to AAAAA)PromoterAvailable sinceJan. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pLenti puro HA-H2BC11
Plasmid#227588PurposeLentiviral plasmid expressing human H2BC11 proteinDepositorInsertH2BC11 (H2BC11 Human)
UseLentiviralTagsHAExpressionMutationPromoterCMVAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
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