Skip to main content
Addgene
Showing: 941 - 960 of 49244 results

  1. psiCHECK2-miR-194-5p

    Plasmid
    #135720
    Purpose
    Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-194-5p)
    Depositor
    Florian Karreth
    Insert
    miR-194-5p reverse complement
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    135720-plasmid-map-sequence-id-322197

    Enlarge

  2. pUPD2 ER-ER (GB2666)

    Plasmid
    #160598
    Purpose
    two repeats of ER (Estradiol receptor)
    Depositor
    Diego Orzaez
    Insert
    ER-ER
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160598-plasmid-map-sequence-id-322068

    Enlarge

  3. MT4

    Plasmid
    #105711
    Purpose
    Bacterial expression plasmids for production of recombinant metallothionein 4
    Depositor
    Artur Krężel
    Insert
    Metallothionein 4 (MT4 Human)
    Use
    Tags
    Chiting Binding Domanin-Intein
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    105711-plasmid-map-sequence-id-203723

    Enlarge

  4. M70 pGL3 Sp5l P1203

    Plasmid
    #17208
    Depositor
    Randall Moon
    Insert
    Sp5l promoter (sp5l Zebrafish)
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    Promoter
    Available since
    May 18, 2009
    Availability
    Academic Institutions and Nonprofits only
    17208-plasmid-map-sequence-id-8541

    Enlarge

  5. pJAT74

    Plasmid
    #204296
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    204296-plasmid-map-sequence-id-406186

    Enlarge

  6. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid
    #159912
    Purpose
    Mutagenesis of Kcnd3
    Depositor
    Larry Zweifel
    Insert
    Kcnd3 (Kcnd3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159912-plasmid-map-sequence-id-317319

    Enlarge

  7. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

    Plasmid
    #159904
    Purpose
    Mutagenesis of Kcnn3
    Depositor
    Larry Zweifel
    Insert
    Kcnn3 (Kcnn3 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159904-plasmid-map-sequence-id-317308

    Enlarge

  8. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

    Plasmid
    #159916
    Purpose
    Mutagenesis of Penk
    Depositor
    Larry Zweifel
    Insert
    Penk (Penk Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159916-plasmid-map-sequence-id-317327

    Enlarge

  9. pAAV-hSyn-Cre-P2A-dTomato

    Plasmid
    #107738
    Purpose
    Can be used to express Cre recombinase and simultaneously the red fluorescent protein dTomato. Can also be used to create adeno-associated virus for delivery of these genes.
    Depositor
    Rylan Larsen
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Cre, dTomato
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107738-plasmid-map-sequence-id-207220

    Enlarge

  10. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

    Plasmid
    #159918
    Purpose
    Mutagenesis of Drd2
    Depositor
    Larry Zweifel
    Insert
    Drd2 (Drd2 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159918-plasmid-map-sequence-id-317332

    Enlarge

  11. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

    Enlarge

  12. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

    Enlarge

  13. pAAV-SCP1-dSa VPR mini.-2X snRP-1 BsaI gRNA

    Plasmid
    #99698
    Purpose
    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA with BsaI cloning sites for programming, vector allows for strong activation of programmed target gene, can be packaged and delivered as AAV
    Depositor
    George Church
    Type
    Empty backbone
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    99698-plasmid-map-sequence-id-194602

    Enlarge

  15. pET22-Nsp10-TEV-6His

    Plasmid
    #154957
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154957-plasmid-map-sequence-id-306327

    Enlarge

  16. pBabe puro IkB alpha M

    Plasmid
    #12332
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Promoter
    Available since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  17. pELK3.1.0-gDNA

    Plasmid
    #112418
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ELK3
    Depositor
    Kevin White
    Insert
    ELK3 (ELK3 Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112418-plasmid-map-sequence-id-230297

    Enlarge

  18. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

    Enlarge

  19. ANO10 (TMEM16KA-c001)

    Plasmid
    #110267
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TMEM16K (ANO10 Human)
    Use
    Tags
    TEV-His10-Flag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    April 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    110267-plasmid-map-sequence-id-358733

    Enlarge

  20. pAAV-Ef1a-DIO-Synaptophysin-GCaMP6s

    Plasmid
    #105715
    Purpose
    In the presence of Cre, the plasmid can be used to express GCaMP6s fused to the presynaptic protein synaptophysin. Drives enriched expression of GCaMP6s in neuronal axon boutons.
    Depositor
    Rylan Larsen
    Has Service
    AAV5
    Insert
    synaptophysin-GCaMP6s
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Ef1a, Human elongation factor-1 alpha
    Available since
    April 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    105715-plasmid-map-sequence-id-207274

    Enlarge

Showing: 941 - 960 of 49244 results