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Addgene
Showing: 941 - 960 of 1937 results

  1. C106m

    Plasmid
    #121009
    Purpose
    MoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    sfYFP-DAS ssrA (has bbsI)
    Use
    Synthetic Biology
    Tags
    DAS degradation tag for ssrA
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121009-plasmid-map-sequence-id-236172

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  2. C109m

    Plasmid
    #121011
    Purpose
    MoClo golden gate assembly CD part for mfLon-pdt3 (mfLon protease with self-targeting degradation tag pdt3). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    mfLon protease with pdt3 tag
    Use
    Synthetic Biology
    Tags
    pdt3 mfLon degradation tag
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121011-plasmid-map-sequence-id-236323

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  3. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

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  4. T7m

    Plasmid
    #121030
    Purpose
    MoClo golden gate assembly DE part for tracrRNA terminator (natural terminator from S. pyogenes CRISPR-system tracrRNA). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    S. pyogenes tracrRNA terminator
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121030-plasmid-map-sequence-id-236182

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  5. T13m

    Plasmid
    #121032
    Purpose
    MoClo golden gate assembly DE part for ECK120033737 (313x-strength natural terminator; see DOI: 10.1038/nmeth.2515). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    ECK120033737 (second strongest natural Voigt terminator)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121032-plasmid-map-sequence-id-236342

    Enlarge

  6. UC20m

    Plasmid
    #121040
    Purpose
    MoClo golden gate assembly DE part for gN2 gRNA (guide RNA for S. pyogenes Cas9; sequence designed with NUPACK for stable tracrRNA structure and open targeting region).
    Depositor
    Richard Murray
    Insert
    gN2 Cas9 gRNA UC part
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    121040-plasmid-map-sequence-id-236353

    Enlarge

  7. pPD706 DsRed-Express2-ZF13

    Plasmid
    #138793
    Purpose
    Constitutive expression of DsRed-Express2-ZF13 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF13
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138793-plasmid-map-sequence-id-320376

    Enlarge

  8. pPD707 DsRed-Express2-ZF14

    Plasmid
    #138794
    Purpose
    Constitutive expression of DsRed-Express2-ZF14 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF14
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138794-plasmid-map-sequence-id-320377

    Enlarge

  9. pPD708 DsRed-Express2-ZF21

    Plasmid
    #138795
    Purpose
    Constitutive expression of DsRed-Express2-ZF21 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF21
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138795-plasmid-map-sequence-id-320733

    Enlarge

  10. pPD709 DsRed-Express2-ZF36

    Plasmid
    #138796
    Purpose
    Constitutive expression of DsRed-Express2-ZF36 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF36
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138796-plasmid-map-sequence-id-320735

    Enlarge

  11. pPD710 DsRed-Express2-ZF42

    Plasmid
    #138798
    Purpose
    Constitutive expression of DsRed-Express2-ZF42 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF42
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138798-plasmid-map-sequence-id-320741

    Enlarge

  12. pPD711 DsRed-Express2-ZF54

    Plasmid
    #138800
    Purpose
    Constitutive expression of DsRed-Express2-ZF54 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF54
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138800-plasmid-map-sequence-id-320744

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  13. pPD712 DsRed-Express2-ZF55

    Plasmid
    #138801
    Purpose
    Constitutive expression of DsRed-Express2-ZF55 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF55
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138801-plasmid-map-sequence-id-320747

    Enlarge

  14. pPD713 DsRed-Express2-ZF62

    Plasmid
    #138802
    Purpose
    Constitutive expression of DsRed-Express2-ZF62 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF62
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138802-plasmid-map-sequence-id-320750

    Enlarge

  15. pPD160 DsRed-Express2-ZFi92

    Plasmid
    #138803
    Purpose
    Constitutive expression of DsRed-Express2-ZFi92 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZFi92
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138803-plasmid-map-sequence-id-320782

    Enlarge

  16. pPD714 DsRed-Express2-ZF93

    Plasmid
    #138804
    Purpose
    Constitutive expression of DsRed-Express2-ZF93 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF93
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138804-plasmid-map-sequence-id-320785

    Enlarge

  17. pPD260 DsRed-Express2-ZFi97

    Plasmid
    #138805
    Purpose
    Constitutive expression of DsRed-Express2-ZFi97 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZFi97
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138805-plasmid-map-sequence-id-320787

    Enlarge

  18. pPD715 DsRed-Express2-ZF129

    Plasmid
    #138806
    Purpose
    Constitutive expression of DsRed-Express2-ZF129 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF129
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138806-plasmid-map-sequence-id-320788

    Enlarge

  19. pPD716 DsRed-Express2-ZF150

    Plasmid
    #138807
    Purpose
    Constitutive expression of DsRed-Express2-ZF150 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF150
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138807-plasmid-map-sequence-id-320790

    Enlarge

  20. pPD717 DsRed-Express2-ZF151

    Plasmid
    #138808
    Purpose
    Constitutive expression of DsRed-Express2-ZF151 under the CMV promoter.
    Depositor
    Joshua Leonard
    Insert
    DsRed-Express2-ZF151
    Use
    Synthetic Biology
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    138808-plasmid-map-sequence-id-320928

    Enlarge

Showing: 941 - 960 of 1937 results