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pTriEx4-ADCY6-C1(306-672 aa)

Plasmid

#113903

Purpose

His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)

Use

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7, CMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot1iso1-IKGRQtoDSYAA-shRNAres_W

Plasmid

#147900

Purpose

Mammalian Expression of HsNot1iso1-IKGRQtoDSYAA-shRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot1iso1-IKGRQtoDSYAA-shRNAres (CNOT1 Human)

Use

Tags

GFP (G4C mutation)

Expression

Mammalian

Mutation

NM_016284.4, I1423D, K1426S, G1451Y, R1458A, and…

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-H58AF60AA64YV71Y-shRNA1res_W

Plasmid

#147902

Purpose

Mammalian Expression of HsNot9-H58AF60AA64YV71Y-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-H58AF60AA64YV71Y-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR205AH208AR244A-shRNA1res_W

Plasmid

#147907

Purpose

Mammalian Expression of HsNot9-Y203AR205AH208AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR205AH208AR244A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-R205AH208A-shRNA1res_W

Plasmid

#147903

Purpose

Mammalian Expression of HsNot9-R205AH208A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-R205AH208A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot9-Y203AR244A-shRNA1res_W

Plasmid

#147908

Purpose

Mammalian Expression of HsNot9-Y203AR244A-shRNA1res

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

HsNot9-Y203AR244A-shRNA1res (CNOT9 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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LI E-F 35S term c1

Plasmid

#54442

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

Terminator of Cauliflower mosaic virus CaMV

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187

Plasmid

#139309

Purpose

N-terminal half of split PI-PLC targeted to mitochondria

Depositor

Gerry Hammond

Article

Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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LI D-E mCherry + link c1

Plasmid

#54420

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

16 aa GS linker + mCherry

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D destab GFP c1

Plasmid

#54454

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

destablized GFP

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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LI C-D T-Saphire c1

Plasmid

#54416

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Insert

T-saphhire

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Plasmid

#222993

Purpose

Mammalian expression of iGeoCas9(C1) construct with EGFP-targeting sgRNA

Depositor

Jennifer Doudna

Article

Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

Insert

HuEx-2xNLS-iGeoCas9(C1)-2xNLS_EGFP-g1

Use

CRISPR

Tags

Puro

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pTagBFP-C1-FKBP-PIP5K1C-HD-EE

Plasmid

#220090

Purpose

Recruitable PIP5K to make PIP2

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PIP5K1C (PIP5K1C Human)

Use

Tags

TagBFP-FKBP1A(3-108)

Expression

Mammalian

Mutation

amino acids 1-635; D101R, R304D, R445E, K446E

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNES-EGFP-C1-flex-PABD-Spo20

Plasmid

#220081

Purpose

Spo20-PABD PA biosensor with a flexible linker to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-EGFP-SGGGSGGS

Expression

Mammalian

Mutation

amino acids 51-91

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA-cloning-EF1a-BA

Plasmid

#204623

Purpose

piggyBac vector for crRNA cloning and expression. Blasticidin selectable.

Depositor

Akitsu Hotta

Article

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsPAN3_245-550_U

Plasmid

#147694

Purpose

Mammalian Expression of HsPAN3_245-550

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

HsPAN3_245-550 (PAN3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsPAN3_245-687_U

Plasmid

#147695

Purpose

Mammalian Expression of HsPAN3_245-687

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

HsPAN3_245-687 (PAN3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-V5-SBP-C1-HsPAN3_551-687_U

Plasmid

#147696

Purpose

Mammalian Expression of HsPAN3_551-687

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

HsPAN3_551-687 (PAN3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del344-380_T

Plasmid

#147629

Purpose

Mammalian Expression of DmNot2-del344-380

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del344-380 (Rga Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot2-del380-404_T

Plasmid

#147630

Purpose

Mammalian Expression of DmNot2-del380-404

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del380-404 (Rga Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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