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Plasmid#64360Purposebicistronic expression of mRFP-Utrophin and Neo resistanceDepositorInsertmRFP-Utrophin (UTRN Human)
UseTagsIRES-Neo and mRFPExpressionMammalianMutationQ236R; aa1-261 Utrophin presentPromoterCMVAvailable sinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3 containing mRFP1
Plasmid#118082PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertpromoter, RBS, mRFP1
UseEscherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR6K-GFP-RFP
Plasmid#199652PurposeR6K backbone with Golden Gate (GG) cloning sitesDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterJ23119 promoterAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
csr-1_son-1_iRFP670
Plasmid#191749PurposeTest fluorescent properties in Neurospora crassa. Nuclear envelope fluorescent reporter.DepositorInsertNCU04288 (NCU04288 Neurospora crassa)
UseTagsNCU04502 promoter and iRFP670ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-mtIF3 shRNA_TagRFP657
Plasmid#182367PurposeshRNA targeting mtIF3 mRNADepositorInsertmtIF3 (Mtif3 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterU6 promoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-miRFP670nano3
Plasmid#207680PurposeEmpty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-miRFP670nano3 cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
UseTagsiRFP713ExpressionMammalianMutationPromoterAvailable sinceDec. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-CAG-emiRFP703
Plasmid#197205PurposeExpresses the protein of emiRFP703 in mammalian cellsDepositorInsertemiRFP703
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO FAAA)3
Plasmid#130703PurposeMammalian expression plasmid for negative control of mCherry-(SUMO)3.DepositorInsert3 copies of human SUMO1 separated by (GGS)4
UseTagsExpressionMammalianMutationFKVK sequence in each SUMO1 module changed to FAAAPromoterCMVAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSET his-mRFP
Plasmid#113553PurposeFor bacterial expression and purification of mRFPDepositorInserthis-mRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hNR2F2-RFP
Plasmid#22926DepositorInserthuman nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress (NR2F2 Human)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-miRFP
Plasmid#108416PurposeMonomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmidDepositorInsertmiRFP
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
piRFP-FRB-PMP-C-10
Plasmid#139316Purposeperoxisome-resident FRB for chemically induced dimerizationDepositorInsertiRFP: MTOR(2021-2113):[GGSA]2QASNSAVSGLRSGSSGG:PXMP(2-195)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-GG15-irFP
Plasmid#159591PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at GG15
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
LaM8-AK74-irFP
Plasmid#159592PurposeOpto-NanobodyDepositorInsertLaM8 with AsLOV2 at AK74
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV-SYN_mCyRFP2-CREB
Plasmid#137008PurposeAAV backbone, mCyRFP2-CREB donor for R-CREBDepositorInsertCREB1 (Creb1 Rat)
UseAAVTagsmCyRFP2ExpressionMammalianMutationPromoterhSynapsinAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTRE:iRFP670-EFS:Cre-2A-GFP
Plasmid#68477PurposeInducible lentivirus expressing iRFP670, Cre, and GFPDepositorInsertsiRFP670
Cre-2A-GFP
UseLentiviralTagsExpressionMammalianMutationPromoterEFS and pTREAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-22:CETN2-mTagRFP-T
Plasmid#101781PurposeHomology arms and mTagRFPT-linker sequence for N-terminus tagging of human CETN2DepositorInsertCETN2 Homology Arms with mTagRFPT-linker (CETN2 Human)
UseCRISPR; Donor templateTagsmTagRFPT-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceOct. 31, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFX-H2B-iRFP
Plasmid#175842PurposeExpress iRFP with nucleus-targeting sequence in mammalian cellsDepositorInsertiRFP with nucleus-targeting sequence
UseTagsH2B (human histone 2B)ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF1-tagRFP-T
Plasmid#103811PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only