Addgene

We narrowed to 3,885 results for: KRAS

Showing: 941 - 960 of 3885 results

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pMXs-hs-NRAS

Plasmid

#52728

Purpose

retroviral expression of human NRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

NRAS (NRAS Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-EGFP-Rac1(wt)

Plasmid

#13719

Depositor

Klaus Hahn

Article

Kraynov et al Science. 2000 Oct 13. 290(5490):333-7.

Insert

EGFP-Rac1(wt) (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 28, 2007

Availability

Academic Institutions and Nonprofits only

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pSL3287

Plasmid

#139750

Purpose

Base Cas12a CRISPRi plasmid

Depositor

Himadri Pakrasi

Article

Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Blast

Plasmid

#175270

Purpose

MegaGate destination with C-terminal HA tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pFH54

Plasmid

#128183

Purpose

Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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PB-hEF1a-MG

Plasmid

#175504

Purpose

All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

hEF1a

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-HA-Hygro

Plasmid

#175268

Purpose

MegaGate destination with C-terminal HA tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Blast

Plasmid

#175271

Purpose

MegaGate destination with C-terminal DD tag and blasticidin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-EF1a-MegaGate-DD-Hygro

Plasmid

#175267

Purpose

MegaGate destination with C-terminal DD tag and hygromycin resistance

Depositor

George Church

Article

Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

Type

Empty backbone

Use

Tags

Destabilization Domain

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pKL11-GCaMP6f bb100

Plasmid

#158983

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f

Use

Tags

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23100 (sigma 70)

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCRISPRi-D2

Plasmid

#182927

Purpose

inducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803

Depositor

Himadri Pakrasi

Article

Liu et al ACS Synth Biol. 2020 Jun 19;9(6):1441-14

Insert

ddcpf1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pFH55

Plasmid

#125814

Purpose

Level0 nSpCas9-PmCDA, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nSpCas9-PmCDA, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH35

Plasmid

#126009

Purpose

Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Arabidopsis AtU6-26 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH33

Plasmid

#125885

Purpose

Level0 Wheat TaU3 Pol III promoter with transcription Start-A

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Wheat TaU3 Pol III promoter with transcription Start-A

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH23

Plasmid

#128180

Purpose

Level1 ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SpCas9 (wheat codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH53

Plasmid

#128182

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH24

Plasmid

#131763

Purpose

Level0 SpCas9, wheat codon optimized (version 2)

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SpCas9, wheat codon optimized, version 2

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pFH58

Plasmid

#128184

Purpose

Level1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH92

Plasmid

#125820

Purpose

Level0 nScCas9-ABE7.10, wheat/human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9-ABE7.10, wheat/human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSaI14

Plasmid

#8614

Depositor

Robert Hartley

Article

Krajcikova et al J Bacteriol 1998 Mar;180(6):1582-5.

Insert

Ribonuclease inhibitor SaI14

Use

Tags

Expression

Bacterial

Mutation

N terminal threonine changed to alanine

Promoter

Available since

Jan. 17, 2007

Availability

Academic Institutions and Nonprofits only