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pB_tetO7_sfGFP

Plasmid

#196616

Purpose

Plasmid for integrating tetO7-controlled sfGFP into the HO region in yeast

Depositor

Matthias Heinemann

Article

Takhaveev et al Nat Metab. 2023 Feb;5(2):294-313. doi: 1

Insert

Superfolder GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

tetO7 promoter

Available since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_SaCRISPR_GFP

Plasmid

#118636

Purpose

To clone sgRNA for SaCas9 (SauCas9)

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_KMT2A_MLLT10

Plasmid

#205843

Purpose

Express mEGFP-tagged fusion protein, KMT2A_MLLT10 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KMT2A_MLLT10 (KMT2A, MLLT10 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pBItetDT960GFP

Plasmid

#80419

Purpose

tet inducible bidirectional plasmid expressing GFP in one direction and in the other a human DMPK genomic segment containing exons 11-15 with 960 interrupted CTG repeats in exon 15 located

Depositor

Thomas Cooper

Article

Lee et al Proc Natl Acad Sci U S A. 2012 Mar 13;10

Insert

EGFP and human DMPK exons 11-15 with 960 interrupted CTG repeats (DMPK Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2016

Availability

Academic Institutions and Nonprofits only

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ERroGFP_iE_pCDNA3

Plasmid

#47954

Purpose

Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

ERroGFP

Use

Tags

FLAG

Expression

Mammalian

Mutation

insertion E147B

Promoter

Available since

Oct. 8, 2013

Availability

Academic Institutions and Nonprofits only

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pCDH_blast_MCS_Nard_GFP_D50

Plasmid

#167339

Purpose

Expresses GFP fused to progerin (d50) in mammalian cells

Depositor

Tom Misteli

Article

Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

Insert

Progerin

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

50AA deletion creating Progerin instead of mature…

Promoter

CMV

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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WT-eGFP

Plasmid

#215168

Purpose

Encodes the enhanced green fluorescent protein

Depositor

Bhuvana Shanbhag, Travis Beddoe

Insert

WT-eGFP

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 lac

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD_sfGFP3TCG4TAG

Plasmid

#174518

Purpose

Recoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.

Depositor

Jason W Chin

Article

Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

Insert

sfGFP

Use

Tags

6 x His

Expression

Bacterial

Mutation

Recoded. TCG and TAG codons were introduced in po…

Promoter

araBAD

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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3XHalo_GFP_TRF1#1

Plasmid

#164644

Purpose

tag TRF1 with GFP and Halo

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

TRF1 (TERF1 Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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EF.GFP

Plasmid

#17616

Depositor

Linzhao Cheng

Article

Cui et al Blood. 2002 Jan 15. 99(2):399-408.

Insert

EF-1a promoter (EEF1A1 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pCAGFP

Plasmid

#32748

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Insert

GFP

Use

Tags

DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

Expression

Mammalian

Mutation

S65T

Promoter

CMV

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pILGFPE9

Plasmid

#83545

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBacMam3_N_His8_eGFP_3C

Plasmid

#178807

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Use

Tags

His8, eGFP, 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pColE1_70a_deGFP_AmpR

Plasmid

#119161

Purpose

Expresses deGFP from a strong sigma 70 promoter

Depositor

Vincent Noireaux

Article

Collias et al Synth Biol (Oxf). 2019 Jan 21;4(1):ysz00

Insert

deGFP

Use

Crispr

Tags

Expression

Mutation

Promoter

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
pHR_D17_hrGFP

Plasmid

#84616

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into D17 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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PA-GFPmt

Plasmid

#19989

Depositor

Orian Shirihai

Article

Twig et al Am J Physiol Cell Physiol. 2006 Jul . 29

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only
EGFP-AR

Plasmid

#111215

Purpose

fluorescent fusion protein

Depositor

Johannes A. Schmid

Insert

AR (Ar Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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GFP-Ub KO

Plasmid

#11934

Depositor

Nico Dantuma

Article

Bergink et al Genes Dev. 2006 May 15. 20(10):1343-52.

Insert

Ubiquitin KO (RBX1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

All 7 lysines of ubiquitin mutated to arginines.

Promoter

Available since

Aug. 7, 2006

Availability

Academic Institutions and Nonprofits only

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AcGFP1-N1

Plasmid

#54705

Purpose

Localization: N1 Cloning Vector, Excitation: 475, Emission: 505

Depositor

Michael Davidson

Type

Empty backbone

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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