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Showing: 941 - 960 of 16262 results

  9. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid
    #114143
    Purpose
    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    114143-plasmid-map-sequence-id-224089

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  10. pRS405 PEX14-VAC8-RFP

    Plasmid
    #166844
    Purpose
    Expresses Peroxisome localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 390 base pairs from the PEX14 ORF
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, PEX14 Budding Yeast)
    Use
    Tags
    PEX14 transmembrane domain and RFP
    Expression
    Yeast
    Mutation
    PEX14 transmembrane domain
    Promoter
    VAC8 promoter
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166844-plasmid-map-sequence-id-329332

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  14. FB007

    Plasmid
    #119705
    Purpose
    Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter gpdA
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    gpdA
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119705-plasmid-map-sequence-id-237147

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  16. pSN-A112C-GpA

    Plasmid
    #191238
    Purpose
    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.
    Depositor
    Alessandro Senes
    Article
    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do
    Insert
    nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
    Use
    Tags
    nuclease A from S. aureus fused to the TM domain …
    Expression
    Bacterial
    Mutation
    Changed Alanine 112 to Cysteine
    Promoter
    T7 promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    191238-plasmid-map-sequence-id-380469

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  17. FB029

    Plasmid
    #119713
    Purpose
    Promoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Promoter paf
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Promoter paf
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119713-plasmid-map-sequence-id-237611

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  18. FB030

    Plasmid
    #119714
    Purpose
    Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    Terminator paf
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119714-plasmid-map-sequence-id-237150

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  19. CD8a-f-CTS-EGFP

    Plasmid
    #86063
    Purpose
    A mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Fibrocystin (Pkhd1 Mouse)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains CD8a luminal and transmembrane domains a…
    Promoter
    CMV
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86063-plasmid-map-sequence-id-177944

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  20. FB005

    Plasmid
    #119678
    Purpose
    Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation
    Depositor
    Jose Marcos
    Article
    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d
    Insert
    geneticin resistance marker
    Use
    Synthetic Biology; Domestication of dna parts for…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    119678-plasmid-map-sequence-id-237143

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Showing: 941 - 960 of 16262 results