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pRS416-PHO5-GFP-Osh3
Plasmid#58839PurposeExpresses GFP-Tagged Osh3 in yeastDepositorInsertOsh3p (OSH3 Budding Yeast)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNM3749
Plasmid#154108PurposeloxP FRT FRT3 RMCE landing site in a Golden Gate SapI GCG ACG assembly vectorDepositorInsertsLoxP-SEC-LoxP
FRT::rpl-28p::GFP::his-58::FRT3
UseCre/Lox; RmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
Omega Destination-CMV::ELuc:bGH
Plasmid#124528PurposeOmega destination vector, that has a CMV:ELUC:bgH in the backboneDepositorTypeEmpty backboneUseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
PH-Gab2-GFP
Plasmid#35147DepositorInsertGab2 PH Domain(x2)
UseTagsEGFPExpressionMammalianMutationContains two copies of the PH domain of Gab2: aa …PromoterCMVAvailable sinceMarch 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120033127
Plasmid#46011DepositorInsertECK120033127 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAGM49981
Plasmid#169087PurposeMoClo level 0 BvADHα coding sequence moduleDepositorInsertBvADHα
UseSynthetic Biology; Moclo cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLAG_OriM
Plasmid#110097PurposeAnalogous to pFLAG_attP, but containing oriM instead of attP, thus being a replicative vector.DepositorTypeEmpty backboneUseTags3xFLAGExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)
Plasmid#50031PurposeExpression of S. pyogenes SrtA at the plasma membrane in mammalian cellsDepositorInsertPM-SrtA(strep)
UseTagsC-HAExpressionMammalianMutationPromoterpCMVAvailable sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSP568
Plasmid#139493PurposeInsertion of the pSTL1 promoter and 24 MS2 stem loops in the GLT1 locus using HIS marker in S. cerevisiaeDepositorInsertpSTL1 24xMS2sl
UseTagsExpressionYeastMutationWTPromoterpSTL1Available sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmDCP1
Plasmid#21684DepositorInsertDecapping protein 1 (Dcp1 Fly)
UseDrosophila expressionTagsEGFPExpressionMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNM3689
Plasmid#154111PurposeminiMos single component RMCE landing site vectorDepositorInsertloxP::SEC::loxP::mex-5p::FLP::sl2::nNeonGreen::glh-2 3'::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3
UseMinimosTagsExpressionMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAGM921
Plasmid#169086PurposeMoClo level 0 Db5GT coding sequence moduleDepositorInsertDb5GT
UseSynthetic Biology; Moclo cloning vectorTagsExpressionMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKOdeltagalK (Hyg)
Plasmid#110090PurposeAnalogous to pKO but without the negative selection gene galK.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSL1433
Plasmid#129523PurposepDEF-SpCas9::GFP, Empty Vector for RGR and HDR Template, PyBiP-minimal promoter, 3rd GenerationDepositorInsertSpCas9 (Other)
UsePlasmodium, rodent-infectious speciesTagsExpressionMutationPromoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pARS3_MUbCAS9_MC
Plasmid#128767PurposeT-DNA binary vector for CRISPR/Cas9 gene editing in rice; has a MCS for cloning gRNADepositorTypeEmpty backboneUseTagsHAExpressionPlantMutationPromoterAvailable sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Tom7
Plasmid#66571PurposeConstitutively express mitochondrial localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsTom7 and mCherryExpressionYeastMutationPhyB portion contains aa's 1-908PromoterpADH1Available sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pT7-EGFP-C1-HsAGO3_F
Plasmid#146316PurposeMammalian Expression of HsAGO3DepositorInsertHsAGO3 (AGO3 Human)
UseTagsExpressionMammalianMutationT25APromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-zDrosha
Plasmid#171540PurposeEncodes Zebrafish Drosha proteinDepositorInsertdrosha (rnasen Zebrafish)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mPrx1 (CT#640)
Plasmid#13961DepositorInsertPrx1 promoter (Prrx1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG505-H2B-frFAST
Plasmid#160056PurposeExpresses H2B-frFAST in mammalian cellsDepositorInsertH2B-frFAST
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCOND (Hyg)
Plasmid#110093PurposeSuicide vector based on the TetR/Pip OFF system.DepositorTypeEmpty backboneUseBacterial knockout generationTagsExpressionMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR Src deltaSH2/3-TagBFP
Plasmid#171033PurposeTagBFP-tagged Src variant lacking SH2 and SH3 domains.DepositorInsertSrc
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAC1819_pCR8-DR-SMN2-2-DR
Plasmid#118653PurposeTransient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway DonorDepositorInsertDR-SMN2-2-DR
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120015170
Plasmid#46006DepositorInsertECK120015170 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-RGS18HIS-hphMX4
Plasmid#26883DepositorInsert18xHis tag
UsePcr tagging yeastTagsExpressionMutationPromoterAvailable sinceDec. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET30-T7-His-sfGFP
Plasmid#180755PurposepET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminatorDepositorInsertsuperfolder green fluorescent protein
UseTagsstrep tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR-U6-gRNA-miniCMV-TdTomato
Plasmid#192656PurposeA plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomatoDepositorInsertTdTomato
UseTagsExpressionMammalianMutationPromoterU6, miniCMVAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
XF1 GAL Smad3
Plasmid#12635DepositorInsertSmad3 (SMAD3 Human)
UseTagsGal4 DBDExpressionMammalianMutationPromoterAvailable sinceOct. 10, 2006AvailabilityAcademic Institutions and Nonprofits only -
CyPet-FKBP-E2
Plasmid#175244PurposeBacterial expression and purification, E2 enzyme (UBC9) for SUMOylation, can be recruited to FRB with rapamycinDepositorInsertUBE2I (UBE2I Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro
Plasmid#61441PurposeDox-inducible MyoD with mRNA tag for smFISHDepositorInsertMyoD (Myod1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010783
Plasmid#46009DepositorInsertECK120010783 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-PML peptide
Plasmid#175246PurposeBacterial expression and purification, low affinity SUMOylation substrateDepositorInsertPML (PML Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
Id2-Halo donor
Plasmid#113116PurposeDonor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Id2 coding sequenceDepositorInsertId2 donor region including homology arms and HaloTag
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK125108723
Plasmid#46008DepositorInsertECK125108723 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRPML3-MYC
Plasmid#18833DepositorInsertTRPML3 (Mcoln3 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
DHFR
Plasmid#64200PurposeExpression of E. coli DHFRDepositorInsertDihydrofolate reductase
UseTagsnoneExpressionBacterialMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC1820_pCR8-DR-RG6-SA-DR
Plasmid#118654PurposeTransient transfection; Expressed DR-RG6-SA-DR CasRx gRNA with preprocessed DR 3'; Gateway DonorDepositorInsertDR-RG6-SA-DR
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR3.1-OPNc PSM5
Plasmid#106330PurposeMammalian expression of mutant osteopontinDepositorInsertOPNc (SPP1 Human)
UseTagsExpressionMammalianMutationOPNc phosphorylation site mutant SGSSEEKQNAVSSEET…PromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJH643
Plasmid#172168PurposeReporter plasmid for pylBCD variant in pBAD backboneDepositorInsertPara sfGFP.3TAG; Plpp pylS; PproK pylT; AraC; Cam
UseTagsExpressionBacterialMutationsfGFP(N39*, N135*, Y151*)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH577
Plasmid#172161PurposepylBCD variant in pBAD backboneDepositorInsertPara (pylBCD 3f2); AraC
UseTagsExpressionBacterialMutationPylB(N61S, E84K, E122K, E178K, G188R, E251D) PylD…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pFBXC3H
Plasmid#49050PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFBXNH3
Plasmid#49049PurposeFX cloning insect cell expression vectorDepositorTypeEmpty backboneUseTags3C protease site (PreScission site) and decaHis (…ExpressionInsectMutationPromoterpolH (polyhedrin)Available sinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT103
Plasmid#25087DepositorInsertPompC-lacZ
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
mOrange pHis-//
Plasmid#53304PurposeEmpty vector to express N-terminal His-mOrange-tagged fusion protein.DepositorTypeEmpty backboneUseTagsHis and mOrangeExpressionBacterialMutationPromoterAvailable sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120010800
Plasmid#46010DepositorInsertECK120010800 Terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPBADAvailable sinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSL1166
Plasmid#129520PurposepDEF-SpCas9::GFP, For deletion of pyalba4, PyBiP-full promoter, 2nd GenerationDepositorInsertSpCas9
UsePlasmodium, rodent-infectious speciesTags3XFLAG-NLSExpressionMutationPromoterAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-SEC16
Plasmid#102676PurposeThe gene SEC16 is driven by the strong promoter TEF. Overexpression of SEC16 increases recombinant protein secretionDepositorInsertSEC16 (SEC16 Budding Yeast)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only
