159,157 results

1. PB-CT3G-CERP2-MG-IRES2-mNeonGreen

   Plasmid

   #175508

   Purpose

   All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (CMVe-hEF1a-driven rtTA and puromycin resistance)

   Depositor

   George Church

   Article

   Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G-mCMV

   Available since

   Oct. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Flag p21 WT

   Plasmid

   #16240

   Depositor

   Mien-Chie Hung

   Article

   Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

   Insert

   p21 (CDKN1A Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 30, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCMV Puro DEST p38KTRmCerulean3

   Plasmid

   #59155

   Purpose

   Lentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDH-CB-iCre-P2A-tdTomato-T2A-Puro

   Plasmid

   #72255

   Purpose

   Express iCre, tdTomato and puromycin resistance gene (puromycin N-acetyl-transferase) under CB (chicken beta-actin + CMV enhancer) promoter

   Depositor

   Kazuhiro Oka

   Insert

   iCre-P2A-tdTomato-T2A-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CB and chicken beta-actin + CMV enhancer

   Available since

   March 1, 2017

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-Cyto-iPASnFR-L-mScarlet3-3xFLAG

   Plasmid

   #225242

   Purpose

   Mammalian expression of iPASnFR-L in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-L
   

   Use

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. Lenti-EFS-EGFP-T2A-Bsd

   Plasmid

   #193198

   Purpose

   Lentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance marker

   Depositor

   Julien Sage

   Article

   Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVSVΔG-eGFP-linker

   Plasmid

   #231707

   Purpose

   Encodes the genome of VSV driven by a T7 polymerase promoter. It lacks the G protein (glycoprotein) and encodes eGFP.

   Depositor

   Ron Geller

   Article

   Marques et al Sci Rep. 2024 Jun 25;14(1):14644. doi: 1

   Insert

   VSV antigenome
   

   Use

   Vsv expression

   Tags

   Expression

   Mammalian

   Mutation

   ΔG (VSV glycoprotein removed)

   Promoter

   T7

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMB80 (R26-CreER)

   Plasmid

   #12168

   Depositor

   Tyler Jacks

   Article

   McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Rosa26 CreER^T2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-neo-cterminal-3HA

   Plasmid

   #102643

   Purpose

   To generate three copies of the HA epitope tag on cDNAs.

   Depositor

   Michael E. Wright

   Type

   Empty backbone

   Use

   Tags

   3x HA (YPYDVPDYA)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCM157

    Plasmid

    #45863

    Depositor

    Mary Lidstrom

    Article

    Marx et al Biotechniques. 2002 Nov;33(5):1062-7.

    Insert

    cre recombinase
    

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ki67-mNeonGreen-IRESpuro2

    Plasmid

    #183737

    Purpose

    Expresses Ki67-mNeonGreen

    Depositor

    Daniel Gerlich

    Article

    Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

    Insert

    MKI67 (MKI67 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA-BRCA1-mCherry

    Plasmid

    #195275

    Purpose

    express mCherry-BRCA1 fusion

    Depositor

    Xiaokun Shu

    Article

    Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

    Insert

    mCherry-BRCA1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. MSCV-opEBNA2-IRES-GFP

    Plasmid

    #220354

    Purpose

    Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    3 x HA tag

    Expression

    Mammalian

    Mutation

    codon-optimised for expression in mammalian cells

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pM-mTagBFP2

    Plasmid

    #228615

    Purpose

    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. SLF063: Lenti-fCAG-jRGECO1a

    Plasmid

    #124775

    Purpose

    For general lentiviral or direct expression of cytosolic jRGECO1a

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    jRGECO1a
    

    Use

    Lentiviral

    Tags

    NES-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

19. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB-2

    Plasmid

    #210388

    Purpose

    All-in-one cumate-based inducible CRISPRi plasmid for use in Streptomyces

    Depositor

    Gilles van Wezel

    Article

    Bai et al ACS Synth Biol. 2023 Oct 6. doi: 10.1021

    Inserts

    dCas9
    CymR
    sgRNA cassette
    

    Use

    CRISPR; Integrative vector for use in streptomyces

    Tags

    Expression

    Mutation

    BsaI-flanked RFP cassette inserted in place of sp…

    Promoter

    SP11 and SP30

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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