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Plasmid#195226PurposeshRNA to canine NHERF1.DepositorInsertNHERF1 (SLC9A3R1 canine)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-cfNHERF2_4
Plasmid#195227PurposeshRNA to canine NHERF2.DepositorInsertNHERF2 (SLC9A3R1 canine)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO-cfNHERF2_5
Plasmid#195228PurposeshRNA to canine NHERF2.DepositorInsertNHERF2 (SLC9A3R1 canine)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pHBJT031
Plasmid#225177PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (AmpR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPSt
Plasmid#203590PurposeBase plasmid for Plant STARR-seq terminator libraries.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT034
Plasmid#225180PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (SmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT032
Plasmid#225178PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (TetR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT033
Plasmid#225179PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (GmR). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT030
Plasmid#225176PurposeLow copy cloning vector with mCherry integrated in the multiple cloning site (Chlr). There is a SmaI immediately upstream of mCherry start codon, which allows for C-terminal mCherry fusions.DepositorTypeEmpty backboneUseSynthetic BiologyTagsmCherryExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT004
Plasmid#225155PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in opposite orientation of lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHBJT003
Plasmid#225154PurposeMedium copy cloning vector for integration of C-terminal FLAG epitope tag. SmaI restriction site immediately upstream of the FLAG epitope. FLAG epitope in same orientation as lac promoter.DepositorTypeEmpty backboneUseSynthetic BiologyTagsFLAG epitope tag (35 bp)ExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-SUMO-sfGFP-WT
Plasmid#212122PurposeFluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertsSUMO-sfGFP-WT
M. alvus Pyl-tRNA(6)
UseTagsHis6 and SUMOExpressionBacterialMutationPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pALS3-Ma-sfGFP-TAG150
Plasmid#212121PurposeFluorescence plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses sfGFP-TAG150 and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.DepositorInsertssfGFP-150TAG
M. alvus Pyl-tRNA(6)
UseTagsHis6ExpressionBacterialMutationN150TAGPromoteraraC and lppAvailable sinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2735 (pSpy0C2)
Plasmid#220279Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2796 (pSpy0C4)
Plasmid#220280Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the sagB locus via homologous recombinationDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaM
Plasmid#224428PurposeExpress AzaM in E. coliDepositorInsertazaM
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a-AzaC
Plasmid#224424PurposeExpress AzaC in E. coliDepositorInsertazaC
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only