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Nxph4-2A-CreERT2 Targeting Vector

Plasmid

#62219

Purpose

Targets CreERT2 to the mouse Nxph4 stop codon

Depositor

Hongkui Zeng

Article

Harris et al Front Neural Circuits. 2014 Jul 10;8:76.

Insert

Nxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Mouse Nxph4

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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1014 pcDNA3 T7 Akt1 K179M T308A S473A

Plasmid

#9031

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M T308A S473A (AKT1 Human)

Use

Tags

Expression

Mammalian

Mutation

K179M T308A S473A

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pmCherry-EXO1a

Plasmid

#111629

Purpose

Expresses human WT Exonuclease 1a (EXO1a) tagged with mCherry in mammalian cells

Depositor

Marco Muzi-Falconi

Article

Sertic et al Proc Natl Acad Sci U S A. 2011 Aug 16;10

Insert

Exonuclease 1a (EXO1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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MAC-CLEC4E

Plasmid

#172412

Purpose

MAC-tagged gene expression

Depositor

Markku Varjosalo

Article

Liu et al Mol Syst Biol. 2021 Nov;17(11):e10396. d

Insert

C-type lectin domain family 4 member E (CLEC4E Human)

Use

Tags

MAC-tag

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Nov. 4, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-TRIM24

Plasmid

#65396

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM24 (TRIM24 Human)

Use

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
902 pLNCX myr HA Akt1 K179M

Plasmid

#9006

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 K179M (AKT1 Human)

Use

Retroviral

Tags

HA and myr

Expression

Mammalian

Mutation

K179M. kinase dead.

Promoter

Available since

Feb. 13, 2006

Availability

Academic Institutions and Nonprofits only

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anti-GFP scFv [N86/38] with sortase tag

Plasmid

#204419

Purpose

Mammalian Expression of anti-GFP scFv with a sortase tag for direct dye conjugation. Derived from hybridoma N86/38.

Depositor

Jeff Lichtman

Article

Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

Insert

Recombinant mouse scFv targeting GFP (Aequorea victoria)

Use

Tags

6xHis tag and Sortase tag

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLex 983-Micu2

Plasmid

#50372

Purpose

Expression in mammalian cells

Depositor

Vamsi Mootha

Article

Plovanich et al PLoS One. 2013;8(2):e55785. doi: 10.1371

Insert

MICU2 (MICU2 Human)

Use

Lentivirus

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only
pCAGGS 3xFLAG Mbnl2

Plasmid

#217032

Purpose

Overexpression of Mbnl2 in mammalian cells

Depositor

Chaolin Zhang

Article

Weyn-Vanhentenryck et al Nat Commun. 2018 Jun 6;9(1):2189. doi: 1

Insert

Mbnl2 (Mbnl2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV10-mFoxP1

Plasmid

#35170

Depositor

Benjamin Blencowe

Article

Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

Insert

Foxp1 (Foxp1 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2012

Availability

Academic Institutions and Nonprofits only
DN2-Gli3 in AINGpCAGGS

Plasmid

#121969

Purpose

strong Gli3 transcriptional activator

Depositor

James Briscoe

Article

Stamataki et al Genes Dev. 2005 Mar 1;19(5):626-41. doi:

Insert

DN2Gli3 (GLI3 Human)

Use

Chick electroporation

Tags

5xmyc

Expression

Mammalian

Mutation

N terminus deletion (nt1-1401)

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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METTL7A pEGFP-N2 (890)

Plasmid

#62017

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

METTL7A (METTL7A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5/FRT/TO HIS HSPH2

Plasmid

#19535

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 70kDa protein 4 (HSPA4 Human)

Use

Tags

His

Expression

Mammalian

Mutation

mutations A381T, T732A, and an insertion of "…

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toru Nakano, Toshinobu Nakamura

Article

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF3185

Plasmid

#144661

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TOX2 (TOX2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAdCMV/V5-DEST-STAT3C-3xFlag

Plasmid

#99264

Purpose

Adenoviral vector encoding Flag-tagged murine STAT3C (constitutive)

Depositor

Alejandro Adam

Article

Alsaffar et al Am J Physiol Cell Physiol. 2018 Jan 10.

Insert

Signal transducer and activator of transcription 3 - constitutive dimer (Stat3 Mouse)

Use

Adenoviral

Tags

3x-Flag

Expression

Mutation

A662C N664C

Promoter

CMV

Available since

Jan. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pmKO-imp α

Plasmid

#200082

Purpose

Expresses mKO-tagged human importinα in mammalian cells

Depositor

Kenji Sugimoto

Article

Nakagawa et al Biosci Biotechnol Biochem. 2012;76(2):38

Insert

importin α (KPNA2 Human)

Use

Tags

mKO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Gcn5

Plasmid

#63706

Purpose

retroviral expression of mouse Gcn5 in mammalian cells

Depositor

Kai Ge

Article

Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

Insert

Gcn5 (Kat2a Mouse)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only
pBMNZ-neo-Flag-TNFR1 L380A

Plasmid

#43949

Depositor

Martin Kluger

Article

D'Alessio et al J Biol Chem. 2010 Jul 30;285(31):23868-7

Insert

Tumor Necrosis Factor Receptor 1 (TNFRSF1A Human)

Use

Tags

Flag*

Expression

Mutation

L380A

Promoter

Available since

April 8, 2013

Availability

Academic Institutions and Nonprofits only
pXJ42-p200 CUX1

Plasmid

#100813

Purpose

Plasmid vector expressing human p200 CUX1 (amino acids 1-1505 of P39880), with an N-terminal Myc tag and a C-terminal HA tag

Depositor

Alain Nepveu

Article

Wilson et al J Biol Chem. 2009 Jan 2;284(1):36-45. do

Insert

p200 CUX1 (CUX1 Human)

Use

Note that this plasmid does not have an sv40 ori

Tags

HA and Myc

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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