Addgene

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Venus wGBD

Plasmid

#85222

Purpose

Venus tagged Cdc-42 sensor

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2016 May 1;27(9):1442-50.

Insert

wGBD (WASL Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

Cdc42 binding domain of N-WASP

Promoter

CMV

Available since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pJRM3 - [BAGp | mScarlet | tbb-2 UTR]

Plasmid

#204616

Purpose

mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | wrmScarlet | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJRM4 - [BAGp | gfp | tbb-2 UTR]

Plasmid

#204617

Purpose

gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[BAGp | gfp | tbb-2 UTR]

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (Frag A)

Plasmid

#16605

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Frag A) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

FragA (XmnI->KpnI). The KpnI site is at -1194 …

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pMOD_A0402

Plasmid

#91009

Purpose

Module A, Promoter: AtUbi10, Gene: AtCas9_dead (D10A + H840A), Terminator: HSP

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9_dead (D10A + H840A)

Use

CRISPR

Tags

Expression

Mutation

D10A, H840A

Promoter

AtUbi10

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585

Plasmid

#174401

Purpose

S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pHis-hMTMR1

Plasmid

#100723

Purpose

Expression of human MTMR1 PH-PTP domain

Depositor

Byung Il Lee

Article

Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

Insert

MTMR1 (MTMR1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 95-607

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Use

Tags

2Strep

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-ARF

Plasmid

#19929

Depositor

Yue Xiong

Article

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pdCas9-omega

Plasmid

#114632

Purpose

Addgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAP

Depositor

Anushree Chatterjee

Article

Otoupal et al Commun Biol. 2018 Sep 3;1:129. doi: 10.1

Insert

dCas9-omega

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Tet promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pKanCMV-CX4.3-7aa-mClover3

Plasmid

#74260

Purpose

Mammalian expression of C-terminal fusion of mClover3 to CX43

Depositor

Michael Lin

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Insert

Connexin 43

Use

Tags

mClover3

Expression

Mammalian

Mutation

Promoter

CAG and CMV

Available since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO CMV-cGFP-bGH Poly(A) Sense

Plasmid

#46835

Purpose

Express cGFP transcript ending in a poly(A) tail [using the bGH poly(A) signal]

Depositor

Phil Sharp

Article

Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

Insert

Coral Green Fluorescent Protein (cGFP)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2013

Availability

Academic Institutions and Nonprofits only

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pBLO2 casX

Plasmid

#123122

Purpose

E. coli expression vector for CasX sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX guide RNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

Plasmid

#40731

Depositor

Daniel Gerlich

Article

Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

Insert

Aurora B FRET-sensor

Use

Tags

CyPet and YPet

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_RACK1

Plasmid

#127123

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA RACK1 (RACK1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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p231-pOsAct1::hpt-pZmUbi::BdPetC-p35S::mTurquoise

Plasmid

#129650

Purpose

Rieske FeS overexpression

Depositor

Susanne von Caemmerer

Article

Ermakova et al Commun Biol. 2019 Aug 16;2:314. doi: 10.

Insert

CDS of PetC gene encoding for the Rieske FeS subunit

Use

Tags

Expression

Plant

Mutation

Codon modified for Golden Gate

Promoter

pZmUbi

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-POLD3

Plasmid

#183197

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-POLD3

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMAX deltaN-ERG

Plasmid

#29447

Depositor

Valeri Vasioukhin

Article

Klezovitch et al Proc Natl Acad Sci U S A. 2008 Feb 12. 1

Insert

ERG (ERG Human)

Use

Tags

Expression

Mammalian

Mutation

Deletion of first 33 aa of ERG

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pOBCol3.6-GFPtpz

Plasmid

#110211

Purpose

Labels immature osteoblasts and connective tissue cells expressing type 1 collagen.

Depositor

David Rowe

Article

Kalajzic et al J Bone Miner Res. 2002 Jan;17(1):15-25.

Insert

eGFPtopaz

Use

Tags

None

Expression

Mammalian

Mutation

None

Promoter

3.6 kb rat type 1 collagen + first intron

Available since

June 18, 2018

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Annexin-12

Plasmid

#55276

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Annexin (A4) (ANXA4 Human)

Use

Tags

mTagBFP2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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