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pcDNA3-CARD14-E138A

Plasmid

#69921

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138A

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)

Plasmid

#71213

Purpose

dCas9 fused to BFP and the human DNMT3A catalytically inactive domain

Depositor

Yonglun Luo

Article

Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

Insert

dSpCas9-BFP-DNMT3A(E752A), DNMT3A catalytic domain with inactivation mutation E752A

Use

Tags

dSpCas9-BFP-DNMT3A(E752A)

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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Lenti-sgEp300#2/Cre

Plasmid

#173592

Purpose

Expresses a Ep300-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Ep300 (Ep300 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgFat1#1/Cre

Plasmid

#173633

Purpose

Expresses a Fat1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Fat1 (Fat1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-G117S

Plasmid

#69920

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G117S

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

Plasmid

#139444

Purpose

Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

sequence codon-optimized

Promoter

SFFV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pMG023 MBP-Axin 384-518-His (miniAxin)

Plasmid

#154012

Purpose

Expresses MBP-Axin_384-518-His (human Axin minimal scaffold fragment as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

Axin1 (AXIN1 Human)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

381-518 fragment

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-124-EF1a-GFP

Plasmid

#117318

Purpose

Overexpression of miR-124 in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-124-1 (MIR124-1 Human)

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Tet-inducible promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCdkn2a#1/Cre

Plasmid

#173629

Purpose

Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2a (Cdkn2a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-sgCdkn2a#2/Cre

Plasmid

#173630

Purpose

Expresses a Cdkn2a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cdkn2a (Cdkn2a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCmtr2#2/Cre

Plasmid

#173632

Purpose

Expresses a Cmtr2-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Cmtr2 (Ftsjd1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-KAE

Plasmid

#120728

Purpose

Expression of a Flag-tagged version of DDX11 KAE mutant in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 KAE mutant (DDX11 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Substitution of E201 and Y202 with K and A

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-DDX11-Flag-KAK

Plasmid

#120729

Purpose

Expression of a Flag-tagged version of DDX11 KAK mutant in mammalian cells

Depositor

Francesca Pisani

Article

Cortone et al PLoS Genet. 2018 Oct 10;14(10):e1007622.

Insert

DDX11 KAK mutant (DDX11 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

Substitution of E201, Y202 and E203 with K, A and…

Promoter

CMV

Available since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgKeap1#2/Cre

Plasmid

#173640

Purpose

Expresses a Keap1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Keap1 (Keap1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826)_FIH

Plasmid

#99508

Purpose

Bacterial expression of hydroxylated Hif1a TAD (776-826)

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
FIH1 (HIF1AN Human)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

Plasmid

#117319

Purpose

Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

hsa-miR-CTRL

Use

Transposon-mediated integration

Tags

GFP

Expression

Mammalian

Mutation

miR-124 deleted

Promoter

Tet-inducible promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgDnmt3a#1/Cre

Plasmid

#173587

Purpose

Expresses a Dnmt3a-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Dnmt3a (Dnmt3a Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-CARD14-D176H

Plasmid

#69919

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

D176H

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
Flag-hPKL S113D

Plasmid

#107161

Purpose

For mammalian expression of Flag-hPKL S113D

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113D (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to aspartate

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-hPKL S113A

Plasmid

#107160

Purpose

For mammalian expression of Flag-hPKL S113A

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

Flag-hPKL S113A (PKLR Human)

Use

Retroviral

Tags

N-term Flag

Expression

Mammalian

Mutation

Serine 113 mutated to alanine

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge