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AAV-CAG-flex-MCS--4x6T

Plasmid

#196420

Purpose

Empty vector for astrocyte-selective Cre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Article

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV and Cre/Lox; Astrocyte-selective

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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PER2:Luc Plasmid

Plasmid

#206875

Purpose

Per2 promoter luciferase reporter

Depositor

Michelle Farkas

Article

Lin et al Integr Cancer Ther. 2019 Jan-Dec;18:1534

Insert

Period 2 (Per2 Mouse)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Use

Tags

8×his

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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PLJR962

Plasmid

#115162

Purpose

Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

Depositor

Sarah Fortune

Article

Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

Inserts

Sth1 sgRNA scaffold
Sth1 dCas9
TetR
L5 attP
L5 Int
KanR

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-Alb-mNG

Plasmid

#183466

Purpose

To produce AAV with the CBh promoter to express Alb(mouse)-mNeonGreen fusion protein

Depositor

Hajime Hirase

Article

Wang et al Cell Rep Methods. 2022 Sep 21;2(10):1003

Insert

Alb (Alb Mouse)

Use

AAV

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

May 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-Sac-Abeta(M1-42)

Plasmid

#71875

Purpose

Expresses the amyloid-beta protein (42 aa), containing an exogenous methionine (Abeta(M1-42)), when expressed in BL21* DE3 p LysS E. coli cells

Depositor

Dominic Walsh

Article

Walsh et al FEBS J. 2009 Mar;276(5):1266-81. doi: 10

Insert

Abeta(M1-42) (APP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

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LbCas12a-niCPE

Plasmid

#213747

Purpose

For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

Depositor

Caixia Gao

Article

Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

MCP-nLbCas12a-M-MLV RT∆RNase H

Use

CRISPR

Tags

BPNLS

Expression

Mammalian

Mutation

D156R, R1138A in LbCas12a

Promoter

CMV

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)

Plasmid

#209079

Purpose

Ngn2 mediated cortical neuron induction, along with sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

sfGFP
NGN2 (Neurog2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

TRE4G

Available since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pENN496_miniCRISPRcharm_dSaCas9

Plasmid

#220837

Purpose

mini CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

mini CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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PRK5-FLAG-Bag6

Plasmid

#61836

Purpose

express N-terminally FLAG tagged human Bag6

Depositor

Yihong Ye

Article

Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

Insert

Bag6 (BAG6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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