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Plasmid#204289PurposeFor marking CRISPR HDR integrant with EGFP expressed in flight muscles.DepositorInsertMHC-EGFP+H3:H26
UseCRISPRTagsExpressionMutationPromoterMHCAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET16b-His-ArgRS
Plasmid#124104PurposeBacterial expression plasmid for PURE system componentsDepositorInsertArgin+I4 ligase (argS Escherichia coli)
UseTags10x-HisExpressionBacterialMutationPromoterAvailable sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGb_PMLlxP_EGFP
Plasmid#140283Purposea targeting plasmid for EGFP-PMLDepositorInsertEGFP
UseMouse TargetingTagsExpressionMutationPromoterendogenousAvailable sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-RF3
Plasmid#124131PurposeBacterial expression plasmid for PURE system componentsDepositorInsertRelease factor 3 (prfC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-MetRS-His
Plasmid#124115PurposeBacterial expression plasmid for PURE system componentsDepositorInsertMethionine--tRNA ligase (metG Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pQE30-His-IF3
Plasmid#124125PurposeBacterial expression plasmid for PURE system componentsDepositorInsertInitiation factor 3 (infC Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBAD-HisD-miniGFP2
Plasmid#188897PurposeExpress 6xHis-Tag miniGFP2 in E.coliDepositorInsertminiGFP2
UseTags6xHisExpressionBacterialMutationPromoteraraBADAvailable sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE26
Plasmid#90368PurposeC/EBPb - CEBPB gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterCEBPbAvailable sinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-ThrRS
Plasmid#124119PurposeBacterial expression plasmid for PURE system componentsDepositorInsertThreonyl-tRNA ligase (thrS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlnRS-His
Plasmid#124108PurposeBacterial expression plasmid for PURE system componentsDepositorInsertGlutaminyl-tRNA ligase (glnS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUC-hrDNA-12.0
Plasmid#206320PurposeA plasmid containing a 12.0 kb EcoRI fragment that corresponds to sequences between 30.5 and 42.5 kb of the human rDNA repeat (GenBank: U13369)DepositorInsertIntergenic spacer of human rDNA repeat
UseCloningTagsExpressionMutationPromoterAvailable sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXTm-PH domain
Plasmid#210326PurposeExpresses EGFP with PH domainDepositorInsertPH domain
UseFish expressionTagsExpressionMutationPromoterAvailable sinceJan. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBR_attB(bxb)_ccdB_lox_v2
Plasmid#198040PurposeBxb1-specific donor plasmid for the cloning of multiple inserts by Golden Gate assembly (BpiI)DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMay 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL-FLKif3A
Plasmid#13742DepositorInsertmouse Kif3A (Kif3a Mouse)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pwtCas9-bacteria
Plasmid#44250PurposeaTc-inducible expression of wild-type Cas9 (S .pyogenes) for bacterial gene knockdownDepositorInsertwild-type Cas9
UseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceApril 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
p15A_SpyCas9_CmR
Plasmid#119162PurposeExpresses S. pyogenes Cas9DepositorInsertCas9 from S. pyogenes (NEWENTRY )
UseCrisprTagsExpressionMutationPromoterAvailable sinceDec. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
GAG-Pan
Plasmid#99640PurposeEGFP-mCherry Met71GAG reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationMet71 mutated to GAG (Glu)PromoterCMVAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT-Pan
Plasmid#99638PurposeEGFP-mCherry control reporter for methionine misincorporationDepositorInsertmCherry
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-NDK
Plasmid#124136PurposeBacterial expression plasmid for PURE system componentsDepositorInsertNucleotide diphosphate kinase (ndk Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only