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Plasmid#112720PurposeWild-type TBP6.7 was one of the first evolved TAR-binding proteins to bind with exceptional affinity. It was this protein that was used to obtain co-crystals with WT TAR RNA.DepositorInsert6His-TEV-TBP6.7(WT)
UseTags6His-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P46A)
Plasmid#112721PurposeThis mutation (P46A) marks the first residue within the evolved region of U1A.DepositorInsert6His-TEV-TBP6.7(P46A)
UseTags6His-TEVExpressionBacterialMutationP46APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q48T)
Plasmid#112725PurposeThis mutant of TBP6.7 binds to TAR very similarly to TBP6.7(WT), with only 1.4-fold reduced Kd.DepositorInsert6His-TEV-TBP6.7(Q48T)
UseTags6His-TEVExpressionBacterialMutationQ48TPromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(P51A)
Plasmid#112728PurposeSimilar to P46, P51 was selected and is present in all evolved TBPs. Mutating this position into Ala has a modest effect on binding.DepositorInsert6His-TEV-TBP6.7(P51A)
UseTags6His-TEVExpressionBacterialMutationP51APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(Q54A)
Plasmid#112730PurposeAlthough not evolved, this residue within TBPs participates in stabilizing the polypeptide loop responsible for RNA recognition.DepositorInsert6His-TEV-TBP6.7(Q54A)
UseTags6His-TEVExpressionBacterialMutationQ54APromoterT7Available sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-2/3
Plasmid#91551PurposeProtein expression and purification of human SH3 domain construct BZRAP1-2/3DepositorInsertBZRAP1-2/3 (TSPOAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF2-2/3
Plasmid#91557PurposeProtein expression and purification of human SH3 domain construct SH3RF2-2/3DepositorInsertSH3RF2-2/3 (SH3RF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NOXO1-2/2
Plasmid#91558PurposeProtein expression and purification of human SH3 domain construct NOXO1-2/2DepositorInsertNOXO1-2/2 (NOXO1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-3/3
Plasmid#91559PurposeProtein expression and purification of human SH3 domain construct BZRAP1-3/3DepositorInsertBZRAP1-3/3 (TSPOAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GRAP-2/2
Plasmid#91529PurposeProtein expression and purification of human SH3 domain construct GRAP-2/2DepositorInsertGRAP-2/2 (GRAP Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PIK3R2-1/1
Plasmid#91535PurposeProtein expression and purification of human SH3 domain construct PIK3R2-1/1DepositorInsertPIK3R2-1/1 (PIK3R2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK2-1/3-SV1/2
Plasmid#91538PurposeProtein expression and purification of human SH3 domain construct NCK2-1/3-SV1/2DepositorInsertNCK2-1/3-SV1/2 (NCK2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MPP4-1/1-IS3/3
Plasmid#91519PurposeProtein expression and purification of human SH3 domain construct MPP4-1/1-IS3/3DepositorInsertMPP4-1/1-IS3/3 (MPP4 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_BZRAP1-1/3
Plasmid#91487PurposeProtein expression and purification of human SH3 domain construct BZRAP1-1/3DepositorInsertBZRAP1-1/3 (TSPOAP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCF2-2/2
Plasmid#91488PurposeProtein expression and purification of human SH3 domain construct NCF2-2/2DepositorInsertNCF2-2/2 (NCF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCF1-2/2
Plasmid#91492PurposeProtein expression and purification of human SH3 domain construct NCF1-2/2DepositorInsertNCF1-2/2 (NCF1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_TJP2-1/1
Plasmid#91493PurposeProtein expression and purification of human SH3 domain construct TJP2-1/1DepositorInsertTJP2-1/1 (TJP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RIMBP2-2/3
Plasmid#91468PurposeProtein expression and purification of human SH3 domain construct RIMBP2-2/3DepositorInsertRIMBP2-2/3 (RIMBP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCF2-1/2
Plasmid#91479PurposeProtein expression and purification of human SH3 domain construct NCF2-1/2DepositorInsertNCF2-1/2 (NCF2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_MYO7A-1/1
Plasmid#91446PurposeProtein expression and purification of human SH3 domain construct MYO7A-1/1DepositorInsertMYO7A-1/1 (MYO7A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only