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Plasmid#128874PurposeFor mammalian expression of mouse Cep83DepositorInsertCep83 (Ccdc41 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BFP
Plasmid#106282PurposeA version of the pHAGE vector with BFP and dsRed expressed separated by an IRES. Note that dsRed is not expressed.DepositorInsertCMV-BFP-IRES-DSRED(NOT-EXPRESSED)-WPRE-MCS
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.3.2
Plasmid#97362PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.3.1
Plasmid#97361PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.3
Plasmid#97358PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.4
Plasmid#97359PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.2.5
Plasmid#97360PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:memGFP
Plasmid#32668PurposeExpresses membrane localized EGFP under the Dusp6 promoterDepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 human cofilin S3A
Plasmid#50854Purposeexpresses constituvely active cofilin in mammalian cellsDepositorInsertcofilin 1 S3A (CFL1 Human)
UseTagsExpressionMammalianMutationconstitutively active, S3APromoterCMVAvailable sinceFeb. 4, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP1 eforRed toehold switch
Plasmid#107353PurposeExpresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressedDepositorInserttoehold switch 1 + eforRed
UseTagsExpressionBacterialMutationPromoterT7Available sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-2-130-FLAG
Plasmid#35984DepositorInserttransactivating protein (tat )
UseTagsFlagExpressionMammalianMutationtat from HIV2 130 amino acidsPromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUC118-mCtnnb1-FLIP-Puro
Plasmid#84540PurposeCRISPR - FLIP targeting vector for mouse beta-cateninDepositorInsertmouse beta-catenin FLIP - Puro targeting vector
UseCre/Lox and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRL CAGpNFlag BAF155 ires GFP
Plasmid#24561DepositorInsertBAF155 (Smarcc1 Mouse)
UseLentiviralTagsFlagExpressionMutationPromoterAvailable sinceJuly 16, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro WT ATG3
Plasmid#26926DepositorInsertautophagy-related 3 (yeast) (Atg3 Mouse)
UseRetroviralTagsHis and V5ExpressionMammalianMutationPromoterAvailable sinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKIF1C-mCherry
Plasmid#130978PurposeExpression of KIF1C-mCherry in mammalian cells.DepositorInsertKIF1C-mCherry (KIF1C Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSG019
Plasmid#126502PurposePseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHisDepositorInsertdisaggregase ClpG with 6xHis-tag
UseTagsC-terminal 6xHisExpressionBacterialMutationPromoterPBADAvailable sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_VP5H
Plasmid#188061PurposePROSS-designed versatile peroxidase 5HDepositorInsertPROSS-designed versatile peroxidase from Pleurotus ostreatus
UseTagsS. cerevisiae native α-factor prepro-leader signa…ExpressionYeastMutation43 PROSS mutations, described in publicationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PlepA
Plasmid#55175PurposeStrong constitutive promoterDepositorInsertlepA promoter
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg1
Plasmid#166998PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only