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pXJ385-pAAV-PB3’IR-U6-gRNA-EF1a-mScarlet-KASH-PB5’IR

Plasmid

#224435

Purpose

AAV piggybac transposon vector, with mScarlet-KASH reporter and gRNA cloning site. Compatible with 10X 5' gRNA capture technology.

Depositor

Xin Jin

Article

Zheng et al Cell. 2024 Jun 20;187(13):3236-3248.e21.

Inserts

mScarlet-KASH
U6-gRNA

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pET-30a (+)_CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

Plasmid

#217014

Purpose

Expression of CDK2-7 chimera in E.coli

Depositor

Manjeet Mukherjee

Article

Mukherjee et al Structure. 2024 Jun 9:S0969-2126(24)0018

Insert

CDK2-7 chimera (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N) (CDK7, CDK2 Human)

Use

Tags

C3 protease cleavable GST tag

Expression

Bacterial

Mutation

CDK2 (I10L,T14Q,V64I,K88E,K89V,D92K,Q131N)

Promoter

Available since

July 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse ZNG1-METAP1 fusion

Plasmid

#197398

Purpose

Expresses a SUMO-tagged fusion of ZNG1, residues 10-30 and mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1-METAP1 (Cbwd1, Metap1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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JDW 771 (pTol2-kdrl:EYFP-hsKRAS4B-WT-ac/Y)

Plasmid

#156413

Purpose

kdrl promoter driving EYFP fused to human KRAS4B with an alpha-crystallin zsYellow reporter flanked by Tol2 sites

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Zebrafish transgenesis

Tags

EYFP

Expression

Mutation

Promoter

kdrl

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 743 (pDONR221-EYFP-hKRAS4B-WT)

Plasmid

#156402

Purpose

Gateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human wild type KRAS4B-WT (KRAS Human)

Use

Gateway cloning

Tags

EYFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 832 (pDONR221-V5-mTagBFP2-KRAS4A-G12D)

Plasmid

#156404

Purpose

Gateway middle entry clone encoding V5-tagged mTagBFP2-fused to human KRAS4A with G12D mutation, includes a stop codon

Depositor

Joshua Wythe

Article

Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

Insert

human mutant KRAS4A-G12D (KRAS Human)

Use

Gateway cloning

Tags

mTagBFP2

Expression

Mutation

mutant KRAS4A-G12D

Promoter

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pEAHISSIRV131

Plasmid

#97053

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 131 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 131

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2462

Plasmid

#97064

Purpose

Expressing Sulfolobus solfataricus orf SSO2462 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2462

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2452

Plasmid

#97059

Purpose

Expressing Sulfolobus solfataricus orf SSO2452 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2452

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPSVORF126

Plasmid

#97021

Purpose

Expressing Pyrobaculum spherical virus orf126 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

PSV orf 126

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA5006

Plasmid

#97016

Purpose

Expressing Pseudomonas aeruginosa orf PA5006 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA5006

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV119

Plasmid

#97054

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 119 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 119

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pDEHISSIRV55

Plasmid

#97055

Purpose

Expressing Sulfolobus islandicus rudivirus (SIRV) orf 55 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

SIRV orf 55

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEANHISECArdB

Plasmid

#96967

Purpose

Expression of E. coli ArdB protein wirh an N-terminal TEV protease cleavable His tag

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

E. coli ArdB

Use

Tags

N-ter TEV cleavable 6His

Expression

Bacterial

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA0856

Plasmid

#97014

Purpose

Expressing Pseudomonas aeruginosa orf PA0856 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

P aeruginosa orf PA0856

Use

Tags

N-ter TEV cleavable 6HIs

Expression

Mutation

N-terminal deletion (AA 1-34)

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPA4631

Plasmid

#97017

Purpose

Expressing Pseudomonas aeruginosa orf PA4631 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Nucleoside-diphosphate-sugar epimerase

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISSSO2373

Plasmid

#97058

Purpose

Expressing Sulfolobus solfataricus orf SSO2373 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO2373

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHISSSO1986

Plasmid

#97057

Purpose

Expressing Sulfolobus solfataricus orf SSO1986 protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

S. solfataricus orf SSO1986

Use

Tags

N-terminal TEV protease cleavable 6xHis tag

Expression

Mutation

none

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsL

Plasmid

#97020

Purpose

Expressing Pseudomonas aeruginosa pqsL protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative FAD-dependent monooxygenase

Use

Tags

N-ter TEV protease cleavable 6HIs

Expression

Mutation

none

Promoter

T7

Available since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAPPFK

Plasmid

#97003

Purpose

Expressing MRSA PPFK protein

Depositor

James Naismith, Huanting Liu

Article

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Putative phosphofructokinase

Use

Tags

N-ter TEV protease cleavable 6His

Expression

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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