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Plasmid#166673PurposeYeast integrative plasmid for expressing wild-type Murine polyomavirus VP1, with GAL1 promoter. Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertWild-type Murine polyomavirus VP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1Available sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnma1a
Plasmid#164951PurposeZebrafish kcnma1a gene CDSDepositorInsertkcnma1a (kcnma1a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNG1304
Plasmid#162488PurposeExpresses HelD K239A from Bacillus subtillisDepositorInsertHelD (helD Bacillus subtillis 168)
UseTags6His-V5ExpressionBacterialMutationChanged Lysine 239 to Alanine (K239A)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsNot4_275-575_AG
Plasmid#148752PurposeMammalian Expression of HsNot4_275-575DepositorInsertHsNot4_275-575 (CNOT4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetDBl-CLIP-HsSAS6
Plasmid#136839PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-Sfi1
Plasmid#136842PurposeMammalian expression of the centrosomal protein hSfi1 N-terminally fused to CLIP-tagDepositorInsertCLIP-hSfi1 (SFI1 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetBl-CLIP-HsSAS6
Plasmid#136838PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to CLIP-tagDepositorInsertCLIP-SAS6 (SASS6 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEBTetD-SNAP-HsSAS6
Plasmid#136790PurposeMammalian expression of the centrosomal protein SAS6 N-terminally fused to SNAP-tagDepositorInsertSNAP-SAS6 (SASS6 Synthetic, Human)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-NR2F2-MUT
Plasmid#138371PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with NR2F2 binding sites mutatedDepositorInsert161k human enhancer with conserved NR2F2 binding sites mutated
UseGateway compatibleTagsExpressionMutationNR2F2 sites mutatedPromoternoneAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5E-161K enhancer-ETS-CORE-MUT
Plasmid#138372PurposeGateway compatible 5' entry clone contains human modified 161k enhancer sequence. with ETS binding sites mutatedDepositorInserthuman 161K human enhancer with ETS bind sites mutated
UseGateway compatibleTagsExpressionMutationETS factor binding sites mutatedPromoterNONEAvailable sinceFeb. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph18
Plasmid#133029PurposeExpresses Xph18 in bacteriaDepositorInsertXph18
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
AcrIIC1-mCherry chimera #10
Plasmid#128113PurposeExpresses a chimeric anti-CRISPR protein targeting the N. meningitidis Cas9DepositorInsertAcrIIC1-mCherry chimera #10
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph20
Plasmid#133024PurposeExpresses Xph20 in bacteriaDepositorInsertXph20
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIGc-Xph15
Plasmid#133030PurposeExpresses Xph15 in bacteriaDepositorInsertXph15
UseTagsHis10ExpressionBacterialMutationS63KPromoterT7Available sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pET28P_dCrk
Plasmid#131145PurposeExpresses 6XHis::dCrk (based on crk isoform RA).DepositorInsertCrk (Crk Fly)
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET15b-GreBdm-D41N
Plasmid#129691Purposeexpress E. coli GreB E82C/C68S/D41NDepositorInsertGreB E82C C68S D41N (greB E. coli)
UseTagshis6ExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSR357.20
Plasmid#125108PurposeExpression of narL(1-154aa)-ydfI(148-213aa) chimera under PLtetO-1, output Promoter PydfJ115DepositorInsertsnarL(REC)-ydfI(DBD)154
sfgfp
tetR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2019AvailabilityAcademic Institutions and Nonprofits only