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Plasmid#194314PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertNGFR-P2A-nLAP(EGFP)
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGTag Hygro EGFP cLAP -1
Plasmid#194312PurposeCRISPR/Cas9-mediated generic protein taggingDepositorInsertcLap(EGFP)-P2A-Hygro
UseBacterial cloning vectorTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
OM14-2EGFP-UG74-ter
Plasmid#184758PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertOM14 (OM14 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SEC7-2EGFP-ter-NAT
Plasmid#184764PurposeMarker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertSEC7 (SEC7 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
IRE1-2EGFP-ter-kanMX4
Plasmid#184766PurposeIntegration of 2xGFP tag at IRE1 C terminus. Potential indicator of UPR activation. Uses antibiotic resistance marker kanMX4.DepositorInsertIRE1 (IRE1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
CIT1-2EGFP-ter-URA3
Plasmid#184767PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at CIT1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertCIT1 (CIT1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
EMC1-2EGFP-ter-NAT
Plasmid#184763PurposeMarker for yeast endoplasmic reticulum (ER). Integration of 2xGFP tag at EMC1 C terminus. Uses antibiotic resistance marker natMX6.DepositorInsertEMC1 (EMC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
ACH1-2EGFP-ter-URA3
Plasmid#184769PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertACH1 (ACH1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
AFG3-2EGFP-ter-URA3
Plasmid#184770PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at AFG3 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertAFG3 (AFG3 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYS1-2EGFP-ter-URA3
Plasmid#184771PurposeMarker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorInsertSYS1 (SYS1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GAL-Aur1-2GFP-Hygromycin
Plasmid#184775PurposeYeast expression of Aur1 under GAL promoter. Uses antibiotic resistance marker hphMX6.DepositorInsertproGAL-Aur1 (AUR1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
UG76-pLYS2-GFP-PKCbeta
Plasmid#184762PurposeMarker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertpLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GLRX-roGFP2 in pT3TS-Dest
Plasmid#194297PurposeIn vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRKDepositorInsertGLRX-roGFP2
UseIn vitro transcription of mrnaTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Rtn2b-RHD272-472-GFP
Plasmid#186606PurposeEncodes human Reticulon 2 isoform B reticulon homology domain aa 272 to 472 fused to GFPDepositorInsertHuman Reticulon 2 isoform B amino acids 272 to 472 (RTN2 Human)
UseTagsmNeonGreenExpressionMammalianMutationMet-Gly-Ser-Lys peptide added at N-terminus for c…PromoterCMVAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
C-SWAT GFP CL1 DAmP
Plasmid#182513PurposeC-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.DepositorInsertCL1 and DAmP
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1630-1706_O
Plasmid#147096PurposeMammalian Expression of HsXRN1_1630-1706DepositorInsertHsXRN1_1630-1706 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_1-132_O
Plasmid#147089PurposeMammalian Expression of HsDCP1a_1-132DepositorInsertHsDCP1a_1-132 (DCP1A Human)
UseTagsExpressionMammalianMutationone silent mutation T237C compared to the sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a_132-582_O
Plasmid#147090PurposeMammalian Expression of HsDCP1a_132-582DepositorInsertHsDCP1a_132-582 (DCP1A Human)
UseTagsExpressionMammalianMutationone silent mutation T1716G compared to the sequen…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1459-1596_O
Plasmid#147093PurposeMammalian Expression of HsXRN1_1459-1596DepositorInsertHsXRN1_1459-1596 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsXRN1_1597-1670_O
Plasmid#147094PurposeMammalian Expression of HsXRN1_1597-1670DepositorInsertHsXRN1_1597-1670 (XRN1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only