Showing: 9461 - 9480 of 145704 results
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Plasmid#140023PurposePlasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integrationDepositorInsertMma PylRS (pylS Methanosarcina mazei)
UseTagsFLAGExpressionMammalianMutationY271A, Y349FPromoterEF1AvailabilityAcademic Institutions and Nonprofits only -
pLARRY-EGFP
Plasmid#140025PurposeLentiviral backbone for cloning expressed barcodes in the 3'UTR of the EGFP mRNADepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEF1alphaAvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro NFIL3 P2A eGFP NLS
Plasmid#140026PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 and enhanced Green Fluorescent Protein (NFIL3 Human, Synthetic)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro eGFP
Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD NFIL3 T2A Puro
Plasmid#140028PurposeTunable and temporal expression control of NFIL3 and puro resistant geneDepositorInsertNFIL3 (NFIL3 Human, Synthetic)
UseSynthetic BiologyTagspuromycinExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro SPI1 P2A mCherry
Plasmid#140029PurposeTunable and temporal expression control of SPI1 and mCherryDepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD NFIL3 P2A eGFP NLS
Plasmid#140030PurposeTunable and temporal expression control of NFIL3 and nuclear eGFPDepositorInsertNFIL3 (NFIL3 Human, Synthetic)
UseSynthetic BiologyTagseGFPExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SPI1 P2A mCherry
Plasmid#140031PurposeTunable and temporal expression control of SPI1 and mCherryDepositorInsertSPI1 (SPI1 Human)
UseSynthetic BiologyTagsmCherryExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
XLone-eGFP NLS (no drug resistant gene)
Plasmid#140032PurposeTunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pSZT025
Plasmid#140033PurposeKO of PCC 7002 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143AvailabilityAcademic Institutions and Nonprofits only -
pSW036
Plasmid#140034PurposeKO of PCC 11901 acsA gene/expresses YFP under control of the cpt promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercptAvailabilityAcademic Institutions and Nonprofits only -
pSW039
Plasmid#140035PurposeKO of PCC 11901 psbA2 gene/expresses YFP under control of inducible cLac143 promoterDepositorInsertYellow fluorescent protein
UseTagsExpressionBacterialMutationPromotercLac143AvailabilityAcademic Institutions and Nonprofits only -
pSW068
Plasmid#140036PurposeKO of PCC 11901 fadD gene/expresses codon-optimized 'tesA gene from E. coli from inducible cLac143 promoterDepositorInsertTruncated thioesterase gene
UseTagsExpressionBacterialMutationPromotercLac143AvailabilityAcademic Institutions and Nonprofits only -
pSW071
Plasmid#140037PurposePCC 11901 fadD locus KO with a kanamycin resistance cassetteDepositorInsertKanamycin resistance gene
UseGene knockout in cyanobacteriaTagsExpressionMutationPromoterKanRAvailabilityAcademic Institutions and Nonprofits only -
pCDF_LuxR
Plasmid#140039PurposeConstutitive expression of LuxR protein.DepositorInsertpLuxL and LuxR
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pGuide-P1-O1a (EL702)
Plasmid#140040PurposeCRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.DepositorInsertbarcode P1 and sgRNA lacO1 array
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pEGFP-2xFYVE
Plasmid#140047PurposeExpresses GFP-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsEGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pmCherry-2xFYVE
Plasmid#140050PurposeExpresses mCherry-tagged HRS 2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain (Hgs Mouse)
UseTagsmCherryExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR
Plasmid#140051PurposeGenetically encoded FRET-based biosensor for monitoring AMPK activity. Also sensitive to phosphorylation by BRSK1/2 when expressed in neurons.DepositorInsertABKAR
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ABKAR(T/A)
Plasmid#140052PurposeNegative control mutant for ABKAR biosensor.DepositorInsertABKAR(T/A)
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 9461 - 9480 of 145704 results