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mVenus-IQ6 WT

Plasmid

#198201

Purpose

FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

MYO5A IQ domain (Myo5a Mouse)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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J23103_GB

Plasmid

#65991

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23103:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23107_GB

Plasmid

#65999

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - medium strength [G:J23107:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23116_GB

Plasmid

#66003

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - low strength [G:J23116:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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J23100_GB

Plasmid

#65983

Purpose

MoClo Basic Part: Constitutive promoter - Anderson series - high strength [G:J23100:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Constitutive promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pSG020

Plasmid

#126503

Purpose

Pseudomonas aeruginosa disaggregase ClpG(GI) cloned as ClpG(GI)-6xHis

Depositor

Ute Romling

Article

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpGgi with 6xHis-tag

Use

Tags

C-terminal 6xHis

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-BaLgp160-754*

Plasmid

#123212

Purpose

Mammalian expression plasmid for Env from the BaL HIV-1 isolate; C-terminal truncation

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (BaL) Env

Use

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; Premature stop co…

Promoter

CMV

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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P445-FBP_17

Plasmid

#139708

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is flower specific, circadian controlled

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pPhODO1:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pYFP-NEAT1_IS_RT

Plasmid

#97091

Purpose

Encodes a HR repair template that mutates the poly(A) signal as well as a few splicing factor binding sites of human NEAT1_1 isoform. Best used with px335-NEAT1_IS_v1 and v2 vectors.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

NEAT1_IS_RT (NEAT1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

CMV

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pvimentin-emiRFP670

Plasmid

#136572

Purpose

Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-emiRFP670

Use

Tags

emiRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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REEP6-EGFP

Plasmid

#210364

Purpose

Expresses REEP6 fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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1456_pDEST_miniTol2_R4-R2_Cryst-eGFP

Plasmid

#171795

Purpose

pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a eGFP selection marker (Crystallin promoter Lens/Eyes)

Depositor

Jean Giacomotto

Article

Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Use

Tags

EGFP

Expression

Insect

Mutation

Promoter

hsp70

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
c-myc promoter (Del 3)

Plasmid

#16603

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (Del 3) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Del-3 (AccI->PvuII). The AccI site is at -741 …

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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px330-UFL1 sgRNA2

Plasmid

#134636

Purpose

contains sgRNA targeting human UFL1 for gene knockout

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

UFL1 sgRNA2 (UFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-Ufm1 ∆C3

Plasmid

#133866

Purpose

Expression of human UFM1 mutant lacking the last three C-terminal residues

Depositor

Yihong Ye

Article

Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

Insert

Ubiquitin-fold modifier 1 lacking last three C-terminal residues (UFM1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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peCALWY-6

Plasmid

#22238

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY6 Zn(II) sensor (ATOX1 GFP's, Human)

Use

Tags

Expression

Mammalian

Mutation

C416S mutation Identical to peCALWY-4, but linke…

Promoter

Available since

Jan. 5, 2010

Availability

Academic Institutions and Nonprofits only
P447-FBP_19

Plasmid

#139710

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway, Luz promoter is ABA responsive

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtuG7, pAtuMas:NnH3H:tAtuMas, p35S:NnHisps:t35S, pAtRAB18:NnLuz:tAtuOcs

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

various

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pUC118-mCtnnb1-FLIP-FlpE-Puro

Plasmid

#84541

Purpose

CRISPR - FLIP-FlpE targeting vector for mouse beta-catenin

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

Insert

mouse beta-catenin FLIP-FlpE - Puro targeting vector

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only