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pnEA-NpM-DmeIF4G_601-660-GB1_AD

Plasmid

#148506

Purpose

Bacterial Expression of DmeIF4G_601-660

Depositor

Elisa Izaurralde

Article

Gruner et al Mol Cell. 2016 Nov 3;64(3):467-479. doi:

Insert

DmeIF4G_601-660 (eIF4G1 Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-DmNanos-opt_116-163-F152E_AB

Plasmid

#148332

Purpose

Bacterial Expression of DmNanos-opt_116-163-F152E

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos-opt_116-163-F152E (nos Fly)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-mut1_G

Plasmid

#146415

Purpose

Mammalian Expression of HsDCP1a-mut1

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

HsDCP1a-mut1 (DCP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmAGO1-RB_B

Plasmid

#145977

Purpose

Insect Expression of DmAGO1-RB

Depositor

Elisa Izaurralde

Article

Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

Insert

DmAGO1-RB (AGO1 Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmCCR4_A

Plasmid

#145894

Purpose

Insect Expression of DmCCR4

Depositor

Elisa Izaurralde

Article

Zekri et al Mol Cell Biol. 2009 Sep 21. ():.

Insert

DmCCR4 (twin Fly)

Use

Tags

Expression

Insect

Mutation

The following variations comprises from our S2 ce…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLV V5-Tmem209

Plasmid

#175154

Purpose

Lentiviral expression of V5-tagged mouse Tmem209

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Tmem209 (Tmem209 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-KR-Linker-eGFP

Plasmid

#176066

Purpose

EGFP fused to the C-terminus of a KR domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

KR

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Zdhhc6-V5

Plasmid

#175146

Purpose

Lentiviral expression of mouse Zdhhc6-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Zdhhc6 (Zdhhc6 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pLV Ptdss2-V5

Plasmid

#175143

Purpose

Lentiviral expression of mouse Ptdss2-V5

Depositor

Larry Gerace

Article

Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

Insert

Ptdss2 (Ptdss2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0233M

Plasmid

#178415

Purpose

Reverse compl seq of pOME0134 flanked by Kpn and EcoRI for insertion into pcDNA3.1-

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

Reverse complement of pOME0134 upstream MIR-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pOME0232M

Plasmid

#178414

Purpose

REVERSE complement seqeunce KSHV Downstream Mir-SYN RNA IN PCDNA3.1-

Depositor

Dirk Dittmer

Article

Alzhanova et al PLoS Pathog. 2021 Jan 7;17(1):e1009033.

Insert

KSHV Reverse complement of downstream MIR-SYN

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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OA-1010A

Plasmid

#176078

Purpose

pBac-10xUAS-L596_g9608-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g9608.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010B

Plasmid

#176079

Purpose

pBac-10xUAS-L596_g25050-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

L596_g25050.t1

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010C

Plasmid

#176080

Purpose

pBac-10xUAS-Ce FAR-8-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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OA-1010D

Plasmid

#176081

Purpose

pBac-10xUAS-Anccey_04371-GGS4-HAx3-p10-UTR-Opie2-dsRed

Depositor

Omar Akbari

Article

Parks et al PLoS Pathog. 2021 Oct 29;17(10):e1010027

Insert

Ce FAR-8

Use

Tags

3xHA

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Ovol1

Plasmid

#172871

Purpose

Mammalian expression vector with myc-tagged mouse Ovol1 coding sequence

Depositor

Renee van Amerongen

Article

Mourao et al bioRxiv 2021.06.22.449461

Insert

Ovol1 (Ovol1 Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-anticalin-FLN-ELP-His-ybbr

Plasmid

#168039

Purpose

Expresses anticalin targeting CTLA-4 with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Anticalin

Use

Tags

3xELP linker, 6x Histag, ddFLN4 (C18S), and ybbr …

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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pFL GST-UBXN2A

Plasmid

#169016

Purpose

Expresses GST-UBXN2A (human) in insect cells

Depositor

Hemmo Meyer

Article

Kracht et al J Mol Biol. 2020 Nov 20;432(23):6061-607

Insert

UBXN2A (UBXN2A Human)

Use

Tags

GST

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-SdrG-FLN-ELP-His-ybbr

Plasmid

#168047

Purpose

Expresses SdrG with FLN fingerprint domain, ELP linker, 6x-His tag and ybbr tag at the C terminus

Depositor

Michael Nash

Article

Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

Insert

Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G

Use

Tags

3xELP linker, 6x Histag, ddFLN4, and ybbr tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMBP-HRV3C-AcrIF9

Plasmid

#141442

Purpose

Expression of AcrIF9 in E. coli, tagged with an HRV3C-cleavage fusion to Maltose Binding Protein.

Depositor

Blake Wiedenheft

Article

Hirschi et al Nat Commun. 2020 Jun 1;11(1):2730. doi:

Insert

AcrIF9

Use

Tags

HRV3C cleavage site and Maltose Binding Protein

Expression

Bacterial

Mutation

Promoter

ptac

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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