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Plasmid#181862PurposeFluoSTEP-AKAR using split CFP and cpVenus(E172) as the FRET donor and acceptor. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.DepositorInsertFluoSTEP-AKAR (C-Y)
UseTagsCFP(1-10) and cpVenus(E127)ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCaf40-A253FY208A_X
Plasmid#147973PurposeInsect Expression of DmCaf40-A253FY208ADepositorInsertDmCaf40-A253FY208A (Rcd-1 Fly)
UseTagsEGFP (A179V)ExpressionInsectMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
UseTagsmCherryExpressionInsectMutationPromoternanosAvailable sinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO/MACROD2
Plasmid#172579PurposeFor generation of FlpIn lines with untagged MACROD2DepositorInsertMACROD2 (MACROD2 Human)
UseTagsnoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HsGIGYF1_1-671_AI
Plasmid#148962PurposeMammalian Expression of HsGIGYF1_1-671DepositorInsertHsGIGYF1_1-671 (GIGYF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X
Plasmid#147993PurposeInsect Expression of DmCaf40-F65AC69YDepositorInsertDmCaf40-F65AC69Y (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC
Plasmid#148466PurposeInsect Expression of DmNanos_116-163-E151AF152AN1555ADepositorInsertDmNanos_116-163-E151AF152AN1555A (nos Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmCaf40_25-291_W
Plasmid#147866PurposeInsect Expression of DmCaf40_25-291DepositorInsertDmCaf40_25-291 (Rcd-1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U
Plasmid#147716PurposeInsect Expression of DmNot2-del458-482-dsRNAresDepositorInsertDmNot2-del458-482-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U
Plasmid#147698PurposeInsect Expression of DmNot2-del393-429-W556E-dsRNAresDepositorInsertDmNot2-del393-429-W556E-dsRNAres (Rga Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NvG-HsNot2_344-540_T
Plasmid#147618PurposeBacterial Expression of HsNot2_344-540DepositorInsertHsNot2_344-540 (CNOT2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsC2orf68-EBMmut_K
Plasmid#146761PurposeMammalian Expression of HsC2orf68-EBMmutDepositorInsertHsC2orf68-EBMmut (C2orf68 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot1iso1_1386-1535_S
Plasmid#147540PurposeMammalian Expression of HsNot1iso1_1386-1535DepositorInsertHsNot1iso1_1386-1535 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLNHA-C1-HsNot1iso1_1318-1605_S
Plasmid#147533PurposeMammalian Expression of HsNot1iso1_1318-1605DepositorInsertHsNot1iso1_1318-1605 (CNOT1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsNot2-F502A_S
Plasmid#147501PurposeMammalian Expression of HsNot2-F502ADepositorInsertHsNot2-F502A (CNOT2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-FLAG-Co-NF-kB
Plasmid#180197PurposeExpresses FLAG-tagged Capsaspora NF-kB protein in mammalian cellsDepositorInsertCo-NF-kB
UseTagsFLAG-tagged fusion proteinExpressionMammalianMutationaa 2-1224PromoterCMVAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-DmeIF4G_601-660-GB1_AD
Plasmid#148506PurposeBacterial Expression of DmeIF4G_601-660DepositorInsertDmeIF4G_601-660 (eIF4G1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpG-DmNanos-opt_116-163-F152E_AB
Plasmid#148332PurposeBacterial Expression of DmNanos-opt_116-163-F152EDepositorInsertDmNanos-opt_116-163-F152E (nos Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsDCP1a-mut1_G
Plasmid#146415PurposeMammalian Expression of HsDCP1a-mut1DepositorInsertHsDCP1a-mut1 (DCP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only