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Plasmid#178343PurposeTo explore endoplasmic reticulum lactate dynamicsDepositorInsertER-CanlonicSF
UseTagsER Retention Signal, ER Signal Exon 1, and ER Sig…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pQCascade-IS3
Plasmid#140626PurposeExpresses V. cholerae TniQ, Cas8, Cas7, and Cas6 and crRNA-IS3. The crRNA-IS3 targets IS3 loci in Escherchia coli.DepositorInsertVchTniQ, VchCas8, VchCas7, VchCas6, crRNA-IS3
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceJuly 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-DR-GC-swap-cr in pBCSK+
Plasmid#126643PurposeCloning vector LbCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertLbCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6 PromoterAvailable sinceJuly 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB65 - pL0_tGFP-I (CDS1)
Plasmid#123182PurposeGolden Gate (MoClo; CDS1) compatible turbo GFP gene from P. plumata with an intron for transient and stable expression in plants (moved to a CDS1 position from pICSL50016; Engler et al., 2014)DepositorInsertturboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
UsePart for plant expressionTagsExpressionPlantMutationNo BpiI and BsaI sites; codon-optimised for plantsPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-RvCAHS8-GS
Plasmid#192461PurposeExpression of RvCAHS8-GS_linker-AcGFP1 in mammalian cellsDepositorInsertCAHS8
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSW554 - pOp5:SP-mVenus-HDEL
Plasmid#116006Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpOp5:SP(ER)-mVenus-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2822 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#106895PurposeExpresses Sp dCas9 and GA-inducible KRAB for NOR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS0035
Plasmid#131125PurposeMammalian Target-AID expression vectorDepositorInsertnCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1 and UGIExpressionMammalianMutationD10A in SpCas9PromoterCMV promoterAvailable sinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA pDEST40 Cas9 GW STOP
Plasmid#183196PurposeDestination vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCIG AKT3 S472E
Plasmid#73053PurposeHuman AKT3 S472EDepositorInsertAKT3 S472E (AKT3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE-BI GFP10-RHOB/RBD-GFP11
Plasmid#182231PurposeInducible coexpression of GFP10-RHOB and RBD-GFP11 domainDepositorInsertsUseLentiviralTagsGFP10 (split-GFP tripartite) and GFP11 (M4)ExpressionMutationPromoterTRE bidirectionalAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ai39
Plasmid#34884DepositorInsertCAG-Floxed eNpHRv3.0-EYFP
UseMouse TargetingTagsEYFPExpressionMutationPromoterCAGAvailable sinceFeb. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTRm1&2
Plasmid#20880DepositorInsertp21 3'UTR site 1 and 2 mutation (Cdkn1a Mouse)
UseLuciferaseTagsExpressionMammalianMutationPredicted miRNA binding site 1 (Position ~420) GC…PromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.hOr47a
Plasmid#126474PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) without N-terminal tags for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsV5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)-V5.mER.hOr47a
Plasmid#126473PurposeHuman codon-optimized version of D. melanogaster Or47a (hOr47a) with an N-terminal tag derived from human HCN1 (mER) for improved intracellular trafficking in mammalian cellsDepositorInsertOr47a (Or47a Fly)
UseTagsH. sapiens HCN1 106VNKFSL111 (mER) and V5 tagExpressionMammalianMutationcodon optimization for H. sapiensPromoterCMVAvailable sinceJune 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVL46 - pWOX4>>
Plasmid#116012Purposedestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expressionDepositorInsertpWOX4:GR-LhG4:tWOX4:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
UseTagsHDEL and SP(ER)ExpressionPlantMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-AS9 (Mito MTS)-Delta N67-mMCL1
Plasmid#45822DepositorInsertAS9(Mito MTS)-Delta N67-mMCL1
UseTagsExpressionMammalianMutationATP Synthase subunit 9 is used as a Mito Targetin…PromoterAvailable sinceJuly 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-ms-c-Myc-L420P
Plasmid#50776Purposeretroviral expression of mouse c-Myc L420P mutantDepositorUseRetroviralTagsExpressionMammalianMutationL420P (does not bind to Max)PromoterAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
attB-GFP-Tet1-Poly(A)
Plasmid#65559PurposePlasmid for Bxb1-mediated recombination of the GFP-Tet1 cDNA into a MIN-tagged locusDepositorInsertTet1 (Tet1 Mouse)
UseMouse Targeting; Bxb1TagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only