Addgene

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Nuclear cpGFP

Plasmid

#215707

Purpose

Expresses cpGFP in the nucleus

Depositor

Katharine Diehl

Article

Smith et al bioRxiv 2023.12.31.573774

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and NLS

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pET His6 GFP TEV LIC cloning vector (1GFP)

Plasmid

#29663

Depositor

Scott Gradia

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

His6-GFP-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pCAX APP Swe/Ind

Plasmid

#30145

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

Insert

human APP 695 Swedish/Indiana mutation (APP Human)

Use

Tags

Expression

Mammalian

Mutation

Swedish and Indiana; K595N, M596L and V642F

Promoter

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-145

Plasmid

#118561

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mScarlet-I

Use

Minitn7 delivery vector

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam APC

Plasmid

#16507

Depositor

Bert Vogelstein

Article

Morin et al Science. 1997 Mar 21. 275(5307):1787-90.

Insert

APC (APC Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only

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pMP31-1

Plasmid

#45905

Depositor

Harmit Malik

Article

Patel et al PLoS Biol. 2012;10(3):e1001282. doi: 10.

Insert

mitochondrial antiviral signaling protein (MAVS Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 19, 2013

Availability

Academic Institutions and Nonprofits only

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pNLS-iRFP670

Plasmid

#45466

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP670

Use

Tags

nuclear localization signal

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1

Plasmid

#175049

Purpose

Overexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5 (RFC4, RFC2, RFC3, RFC5 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)-T2A-Puro

Plasmid

#101039

Purpose

Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

Depositor

Andrea Németh

Type

Empty backbone

Use

CRISPR

Tags

3xFLAG and T2A-Puro

Expression

Mammalian

Mutation

Promoter

CBh

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only

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