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Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gRNA-puro
Plasmid#180426PurposeExpresses puromycin resistance gene and scrambled gRNA for stable integration in mammalian cells; useful as control and template for new gRNAs by mutagenesis.DepositorInsertgRNAscr
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFP-NEAT1m_v1-RT
Plasmid#97089PurposeEncodes a HR repair template for knock-in a YFP expression cassette downstream of the poly(A) signal of human NEAT1_1 isoform. Best used with px330-NEAT1m_v1 vector.DepositorInsertNEAT1m_v1-RT (NEAT1 Human)
UseCRISPRTagsEYFPExpressionMutationPromoterCMVAvailable sinceJuly 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
c-myc promoter (Del 2)
Plasmid#16602DepositorInsertc-myc promoter (Del 2) (MYC Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationDel-2 (KpnI->PvuII). The KpnI site is at -1194…PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-TAT-1-101-FLAG basic mutant
Plasmid#35985DepositorInserttransactivating protein (tat )
UseTagsFlagExpressionMammalianMutationtat from HIV1, 101 aa; R to A mutations at aa 56,…PromoterAvailable sinceSept. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.11.6
Plasmid#97382PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.10.1
Plasmid#97376PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKanCMV-mClover3-18aa-Tubulin
Plasmid#74253PurposeMammalian expression of N-terminal fusion of mClover3 to TubulinDepositorInsertTubulin
UseTagsmClover3ExpressionMammalianMutationPromoterCAG and CMVAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-BeYDV-Rep-RepA-9
Plasmid#197715PurposePhytobrick (MoClo) Level 0 PartDepositorInsertBeYDV Rep/RepA proteins: native ORFs
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10E
Plasmid#91209PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers , Plant Selection: noneDepositorInsert35S:AtCas9 + CmYLCV:gRNAs with tRNA spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
UseTagsTrxA-6xHis-S-tagExpressionBacterialMutationPromoterT7Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(K55M) SpHIS5 (pNTI666)
Plasmid#115436PurposeExpresses K55M RPL28 from HIS3 yeast plasmidDepositorInsertsUseTagsExpressionYeastMutationK55M; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable sinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 NQO1
Plasmid#61730PurposeExpresses WT human NQO1DepositorInsertNAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
UseTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7C-HSPH1
Plasmid#177662PurposeRecombinant protein synthesis of human HSPH1DepositorInsertHSPH1 (HSPH1 Human)
UseTags6xHis-ThrombinExpressionBacterialMutationPromoterT7Available sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB088i
Plasmid#79216PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI(RR69)-TnCAD-F3
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
px330-UFM1 sgRNA2
Plasmid#134634Purposecontains sgRNA targeting human UFM1 for gene knockoutDepositorInsertUFM1 sgRNA2 (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
px330-UFM1 sgRNA1
Plasmid#134633Purposecontains sgRNA targeting human UFM1 for gene knockoutDepositorInsertUFM1 sgRNA1 (UFM1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJ23119-sgRNA
Plasmid#113654PurposesgRNA targeting unit plasmid. The sgRNA targeting unit plasmids contain connector ConLS, ConR1, and one sgRNA transcriptional unit.DepositorInsertpromoter J23119 and sgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterJ23119Available sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Nicastrin-CD4d3+4-bio
Plasmid#73106PurposeEXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bioDepositorInsertNicastrin (NCSTN Human)
UseTagsbiotinylation peptide and ratCD4d3+4ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTagBFP2-Vimentin-7
Plasmid#55333PurposeLocalization: Intermediate Filaments, Excitation: 399, Emission: 456DepositorInsertVimentin (VIM Human)
UseTagsmTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits