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Plasmid#166104PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HCP3
Plasmid#166105PurposeThis plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombinationDepositorInsertMsn2-sgRNA#27 (MSN2 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
J-CDAN1A-5
Plasmid#166522PurposescFv of a human scaffold targeting Codanin-1. Antigen coverage aa 840-1202 of 1227DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Z-OPTN-10
Plasmid#166562PurposescFv of a human scaffold targeting Optineurin. Antigen coverage aa 1-510 of 577DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
peDK.11
Plasmid#112150Purpose"Vector DK"DepositorTypeEmpty backboneUseTagsExpressionMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pSMPUW_8.3_M4_CAMKIIA-gateway-V5-SV40-Zeo
Plasmid#128463Purposelentiviral destination vector with CAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeo Resistance geneDepositorInsertCAMKIIA promoter, C-terminal V5 tag, SV40 promoter, and Zeocin Resistance gene
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJR288
Plasmid#78550PurposeFor expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. Ef1a driven mCherry visible marker.DepositorTypeEmpty backboneUseRNAiTagsExpressionMammalianMutationPromoterU6, EF1aAvailable sinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-L126X-N’His
Plasmid#117707Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationL126XPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q42L-N’His
Plasmid#117708Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ42LPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-Q123A-N’His
Plasmid#117709Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationQ123APromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmSod1-D83G-N’His
Plasmid#117702Purposemutation on stability/solubility of SOD1DepositorInsertSod1 (Sod1 Mouse)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD83GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
phSOD1-D101G-N’His
Plasmid#117704Purposemutation on stability/solubility of SOD1DepositorInsertSOD1 (SOD1 Human)
UseTagsHis tag with Thrombin cleavage siteExpressionBacterialMutationD101GPromoterT7Available sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
TAP-Amotl2
Plasmid#112834Purposetransient/stable overexpressionDepositorInsertAmotl2 (Amotl2 Mouse)
UseTagsTAPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYN-LL5BIP-GFP
Plasmid#112847Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSYN-GFP-LL5BIP
Plasmid#112846Purposetransient/stable overexpressionDepositorInsertLL5BIP (2410002F23Rik Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-mxEH
Plasmid#104977PurposeBiosynthesis of oxylipins by microbial enzymesDepositorInsertMXAN_1644 (MXAN_1644 Myxococcus xanthus)
UseTags6 histidine tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-Vinc(2-258)
Plasmid#80025PurposeMammalian expression of mCherry-vinculin 2-258 (head)DepositorInsertvinculin (VCL Chicken)
UseTagsmCherryExpressionMammalianMutationaa 2-258PromoterCMVAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only