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Plasmid#182675PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.DepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-SFFV-PYL1-sfGFP-Emerin
Plasmid#121532Purposeexpression of PYL-SfGFP-Emerin, lentivirus vectorDepositorInsertEmerin (EMD Human)
UseLentiviralTagsPYL1-SFGFPExpressionMammalianMutationPromoterSFFVAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Emerin
Plasmid#120246PurposeExpresses V5-tagged Emerin in lentiviral vectorDepositorInsertEmerin (Emd Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-TrxF
Plasmid#207454PurposeE. coli expression of Mesembryanthemum crystallinum Thioredoxin F with thrombin-cleavable N-terminal 6xHis tagDepositorInsertThioredoxin F
UseTags6xHisExpressionBacterialMutationCodon optimized for E coli expressionPromoterT7Available sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD066
Plasmid#84032PurposeVector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafishDepositorInsertsCre
Venus
UseCre/Lox; Zebrafish transgenesisTagsmCherryExpressionMutationPromoterCrystalin and ElastaseAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB605-GBSSI
Plasmid#213867PurposeBinary vector for the expression of GBBSI fused to the sGFP in plants (for Agrobacterium-mediated genetic transformation.)DepositorInsertGRANULE-BOUND STARCH SYNTHASE I (GBSSI) (GBSS1 Mustard Weed)
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUltra-U6-gRNAs1-5
Plasmid#178569PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editingDepositorInsert5 gRNA-tRNA array with U6 promoter
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3-Gateway-DSCP
Plasmid#71506PurposeflySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL4-Gateway-SCP1
Plasmid#71510PurposehumanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoterDepositorTypeEmpty backboneUseHumanstarr-seq validation vectorTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV5b HA Irx5 deltaHD
Plasmid#24997DepositorInsertIrx5 (Irx5 Mouse)
UseTagsHAExpressionMammalianMutationDeletes entire HD (aa 112-177)PromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA6.2-YFP-CWT
Plasmid#176689PurposeFluorescent reporter for phagosomal escape of Staphylococcus aureusDepositorInsertcell wall-targeting domain of Lysostaphin
UseTagsYFPExpressionMammalianMutationdeletion of protase domain of LysostaphinPromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV5b HA Irx5 deltaIrot
Plasmid#24995DepositorInsertIrx5 (Irx5 Mouse)
UseTagsHAExpressionMammalianMutationDeletion of Iro box (aa 314-327), 19 aa upstream …PromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pCMV5b HA Irx5 deltaC
Plasmid#24996DepositorInsertIrx5 (Irx5 Mouse)
UseTagsHAExpressionMammalianMutationDeletes everything after Hox Domain (aa 112-177).…PromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
pKrox24(MapERK)d1EGFP (KroxDs)
Plasmid#214912Purposefor PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activationDepositorInsertd1EGFP under the MapERK promoter
UseTagsExpressionMammalianMutationPromoterMapkERK promoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
eGFP-Silicatein-pET28
Plasmid#198012PurposeC-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purificationDepositorInserteGFP-silicatein
UseTagshexahistidine tagExpressionBacterialMutationPromoterT7Available sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW_1
Plasmid#71279PurposeGateway destination vectorDepositorTypeEmpty backboneUseGateway vectorTagsGAL4 DBDExpressionMutationPromoterCMVAvailable sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGE_240_pAGW_V5
Plasmid#71188PurposeGateway destination vectorDepositorTypeEmpty backboneUseGatewayTagsGal4 DBD and V5ExpressionMutationPromoteractin 5CAvailable sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
HI.HslU.RSETB.fl.wt
Plasmid#12556DepositorInsertHaemophilus influenzae heat shock locus U
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
pIMAY
Plasmid#68939PurposeE. coli/staphylococcal temperature-sensitive plasmid for allelic exchangeDepositorTypeEmpty backboneUseStaphylococcal allelic exchange vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSGE_763_4xUAS_UPS_hkCP
Plasmid#71171PurposeLuciferase reporterDepositorInsert763_4xUAS_UPS_hkCP
UseLuciferaseTagsluciferaseExpressionInsectMutationPromoterDSCP Drosophila synthetic core promoterAvailable sinceMay 10, 2016AvailabilityAcademic Institutions and Nonprofits only