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pEGFP-C1-3x-NLS-mScarlet-AMPKα2

Plasmid

#192452

Purpose

mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

Depositor

Jin Zhang

Article

Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

Insert

3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)

Use

Tags

3x Nuclear localization signal (NLS) and mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N3-FLuc-K21-Rluc_AH

Plasmid

#148847

Purpose

Mammalian Expression of K21-Rluc

Depositor

Elisa Izaurralde

Insert

K21-Rluc

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP humanAPOBEC1 L173A/G227A

Plasmid

#167171

Purpose

Expresses human APOBEC1 L173A/G227A in mammalian cells

Depositor

Silvestro Conticello

Article

Chieca et al bioRxiv 2021

Insert

humanAPOBEC1 (APOBEC1 Human)

Use

Tags

Expression

Mammalian

Mutation

changed Leucine 173 to Alanine; changed Glycine 2…

Promoter

CMV promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-30aa-Noxa-4E-pEGFP-C1

Plasmid

#167529

Purpose

To increase the length of the linker between Venus and Noxa-4E proteins

Depositor

David Andrews

Insert

PMA-induced protein 1 (PMAIP1 Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

C25E, L29E, F32E, L36E

Promoter

CMV

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-13aa-Venus-pEGFP-C3

Plasmid

#166753

Purpose

For expression of mCerulean3 linked to Venus by 13 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-13aa-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-79aa-ActA-pEGFP-C1

Plasmid

#166763

Purpose

To target Venus to the mitochondrial outer membrane. ActA tail anchor inserts into the outer membrane and Venus faces the cytosol. Serves as a collisional control in FLIM-FRET experiments.

Depositor

David Andrews

Insert

ActA tail anchor (Listeria monocytogenes) (actA Listeria monocytogenes, Synthetic)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Noxa)-pEGFP-C1

Plasmid

#166761

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Noxa

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Noxa BH3) (Bcl2l11, PMAIP1 Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Noxa…

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-39aa-Venus-pEGFP-C3

Plasmid

#166755

Purpose

For expression of mCerulean3 linked to Venus by 39 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-39aa-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP_∆R13–DD-talin1 (1-2299)

Plasmid

#166125

Purpose

For expression of mouse talin-1 (residues 1-2299) with C-terminal deletion of dimerization domain and R13 (∆R13-DD) in mammalian cells. Contains C-terminal EGFP tag.

Depositor

Vesa Hytönen

Article

Azizi et al Sci Rep. 2021 Jan 11;11(1):347. doi: 10.

Insert

∆R13–DD-Talin-1 (Tln1 Mouse)

Use

Cmv promoter

Tags

EGFP

Expression

Mammalian

Mutation

residues 1-2299, deletion of dimerization domain …

Promoter

Available since

April 13, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean3-31aa-Venus-pEGFP-C3

Plasmid

#166754

Purpose

For expression of mCerulean3 linked to Venus by 31 amino acid linker

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3-31aa-Venus

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bik)-pEGFP-C1

Plasmid

#166760

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bik

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Mus musculus)-(Bik BH3) (Bcl2l11, BIK Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bik …

Promoter

CMV

Available since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bid)-pEGFP-C1

Plasmid

#166759

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bid

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

BimEL(Bid) (Bcl2l11, Bid Mouse)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of mouse Bid …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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Venus-BimEL(Bad)-pEGFP-C1

Plasmid

#166758

Purpose

To examine effect of swapping the BH3 region in full length BimEL with that of Bad

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimEL(Mus musculus)-(Bad BH3) (Bcl2l11, BAD Mouse, Human)

Use

Tags

Venus

Expression

Mammalian

Mutation

BH3 region removed and inserted BH3 of human Bad …

Promoter

CMV

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406A M407A

Plasmid

#112107

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406A M407A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 D406R M407R

Plasmid

#112109

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

D406R M407R

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666E

Plasmid

#105313

Purpose

To study altered downstream functions of phospholylation-site mimetic kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/666E

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP Kind2 S159/666A

Plasmid

#105309

Purpose

To study altered downstream functions of phospholylation-site null kindlin2

Depositor

Kenneth Yamada

Article

Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

Insert

Kindlin2 (FERMT2 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

S159/666A

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-GFP-Ubl4A-N(1-89)

Plasmid

#86922

Purpose

To express Ubl4A (1-89 residues) tagged with FLAG-EGFP at N- terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

UBL4A (UBL4A Human)

Use

Tags

FLAG and GFP

Expression

Mammalian

Mutation

Residues from 90-157 is deleted

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-N2-RIPK3 2KR(89,501)

Plasmid

#78824

Purpose

To detect localization of RIPK3 2KR(89,501) protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K89R, K501R

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-N2-RIPK3 2KR(55,363)

Plasmid

#78823

Purpose

To detect localization of RIPK3 2KR(55,363) protein in live mammalian cells

Depositor

Jaewhan Song

Article

Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

Insert

RIPK3 (RIPK3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K55R, K363R

Promoter

CMV

Available since

June 14, 2016

Availability

Academic Institutions and Nonprofits only