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Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBM6_RRM_pGEX
Plasmid#135157PurposeRecombinant expression of RBM6 with dual-affinity tagDepositorInsertRBM6 (RBM6 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
px335-EF-Slc2a3-R
Plasmid#122305PurposeExpresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cellsDepositorInsertsgRNA for mouse Slc2a3 (Slc2a3 Synthetic)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
ArpC2_pLib
Plasmid#173679PurposeArpC2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorInsertArpC2 (ARPC2 Human)
UseTagsExpressionInsectMutationPromoterpolyhedrinAvailable sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
BOLL_RRM_pGEX
Plasmid#135130PurposeRecombinant expression of BOLL with dual-affinity tagDepositorInsertBOLL (BOLL Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF086 MBP-CREB 127-135-His
Plasmid#154075PurposeExpresses MBP-CREB_127-135-His (human 9aa CREB peptide as a fusion protein with MBP and His- tags) in E.coliDepositorInsertCREB1 (CREB1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterTacAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_Y357F-PolyA
Plasmid#112287PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) Y357F mutant _ corresponding to tyrosine 407 in this isoformDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationTyrosine 357 to Phenyalanine corresponding to tyr…PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-PHD2
Plasmid#223552PurposeLentivirus transfer plasmid for expression of full length human PHD2DepositorInsertPHD2 (EGLN1 Human)
UseLentiviralTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-puro_Panc480-MT7
Plasmid#200941PurposeMultiplexed CRISPR array expressing 7 sgRNA in a lentiviral backboneDepositorInsertmultiplexed sgRNA array
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, EF-1aAvailable sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN491_TALEcharm_MmT8x
Plasmid#220836PurposeTALEcharm Kv2 expression in mammalian cells; targets PrnpDepositorInsertTALEcharm
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENN456_TALEcharm_Kv2_MmT8x
Plasmid#220835PurposeTALEcharm expression in mammalian cellsDepositorInsertTALEcharm Kv2
UseLentiviral and TALENTagsP2A-TagBFPExpressionMammalianMutationN/APromoterEF1aAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMB398_ZFcharm_Prnp_SMM
Plasmid#220845PurposeAAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding siteDepositorInsertZFcharm
UseAAVTagsHAtagExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A pMT2
Plasmid#206110Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
cacna2d1 R241A HA pMT2
Plasmid#206111Purposeexpression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin bindingDepositorInsertcacna2d1 (Cacna2d1 Rat)
UseTagsExpressionMammalianMutationR241APromoterAd MLP/TPL/SV40Available sinceNov. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
p202-Syt1-C2A-I248V
Plasmid#201554PurposeExpresses Octopus synaptotagmin-1 C2A domain with I248V mutation in E. coliDepositorInsertSynaptotagmin-1 C2A domain (partial)
UseTagsExpressionBacterialMutationchanged Isoleucine 248 to Valine (numbering based…PromoterRBSAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1-V5-HisB-ObKHC-K282R-HaloTag
Plasmid#201552PurposeExpresses Octopus kinesin-1 motor with K282R mutation with HaloTag in Drosophila S2 cellsDepositorInsertKinesin-1
UseTagsHaloTagExpressionInsectMutationchanged lysine 282 to arginine (numbering based o…PromoterAc5Available sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS W391A pcDNA3
Plasmid#206077PurposeCav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop that prevents Beta bindingDepositorInsertcacna1b (CACNA1B Rabbit)
UseTagsExpressionMammalianMutationW391APromoterCMVAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199203PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-intDepositorInsertT2A-split-intein-Gal4[1-20]-N-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHD-T2A-split-intein-Gal4[21-881]-C-int
Plasmid#199204PurposeTo create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-intDepositorInsertT2A-split-intein-Gal4[21-881]-C-int
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDropIn-T2A-split-intein-Gal4[1-20]-N-int
Plasmid#199195PurposeInsert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digestDepositorInsertT2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only