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Plasmid#207674PurposeEntry vector for Trio vector system, with vanillate-inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only
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pPEntry
Plasmid#207673PurposeEntry vector for Trio vector system, with DAPG-inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEntry
Plasmid#207672PurposeEntry vector for Trio vector system, with cumate-inducible promoterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
sCatch-GFP-6xHIS
Plasmid#186904PurposeBacterial expression of soluble protein sfGFP-SpyCatcher-sfCherry1-10 with a 6xHIS tagDepositorInsertsfGFP-SpyCatcher003-sfCherry1-10-6xHIS
UseTags6xHis and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
sTag-BFP-6xHIS
Plasmid#186905PurposeBacterial expression of soluble protein tagBFP-sfCherry11-SpyTag with a 6xHIS tagDepositorInsertSpyTag003-sfCherry11-tagBFP-6xHIS
UseTagstagBFP, 6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJH123
Plasmid#172146PurposeMicroscopy inclusion body assayDepositorInsertpCDF.B.(SD4)CD
UseTagsExpressionBacterialMutationPylBCD operon (RBS inserted for PylC)PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
PAJ408
Plasmid#104150PurposedCas9 plasmid expressing full length mKate2 mRNA without RBSDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWDY1228
Plasmid#138594PurposeExpresses NatL2-R156K in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionMutationchanged Arginine 156 to LysinePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHBSTC159
Plasmid#124353PurposePlasmid expressing inactive NpuDnaBΔ283 mini-intein inserted into CcdA antitoxinDepositorInsertNpuDnaBΔ283 (C1G) mini-intein
UseTagsCcdA-C (SFAD)ExpressionBacterialMutationDeletion of 283-residue endonuclease domain, C1GPromoterSTCAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
rpaA_NS1
Plasmid#101829Purposedrives expression of rpaA from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertRpaA with its native promoter
UseCyanobacteria cloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
P0050::rpaA
Plasmid#101830Purposedrives expression of rpaA from an rpaA-independent promoterDepositorInsertRpaA with p0050 promoter (+ gentamicin resistance casette)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
Ptrc::galP_NS1
Plasmid#101843Purposedrives expression of galP transporter from NS1 locusDepositorInsertPtrc promoter driving GalP transporter expression
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGP4GY-cccdB
Plasmid#83878PurposeDestination vector for EYFP fusions integrated at yeast Trp locus, expression from the GPD promoterDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_25
Plasmid#63805Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_14
Plasmid#63804Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_64
Plasmid#63809Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LacI_PlacO1_crtEBI_13
Plasmid#63812Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPlaco1Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_70
Plasmid#63810Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-p85α_wt
Plasmid#39497DepositorInsertp85 (PIK3R1 Bovine)
UseEukaryoticTagsExpressionMutationPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET15b-eIF4E-S200C
Plasmid#37229DepositorInserteIF4E
UseTagsExpressionBacterialMutationS200CPromoterT7Available sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only