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Addgene
Showing: 921 - 940 of 1937 results
  1. L1P137 - pAHP6-PhiC31-NLS-tUBQ1

    Plasmid
    #219702
    Purpose
    Level 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integrase
    Depositor
    Insert
    pAHP6:PhiC31-NLS:tUBQ1
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. Rich-pT7GG

    Plasmid
    #108443
    Purpose
    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS-rich. The vector is compatible for Golden-gate assembly
    Depositor
    Type
    Empty backbone
    Use
    Xenopus oocyte expression system
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. P16m

    Plasmid
    #120940
    Purpose
    MoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    ohAB carb pCinLacO m
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. P32m

    Plasmid
    #120943
    Purpose
    MoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    PlacO (Tim Lu, Matt Bennett, etc)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. P35m

    Plasmid
    #120946
    Purpose
    MoClo golden gate assembly AB part for pLbda-CasRQi (strong lambda promoter with downstream dCas binding site for Qi-lab gRNA (UC16m); gRNA sequence taken from DOI: 10.1016/j.cell.2013.02.022).
    Depositor
    Insert
    pLbda-CasRQi
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. P61m

    Plasmid
    #120953
    Purpose
    MoClo golden gate assembly AB part for pTac (Adam Meyer's improved pTac promoter selected for dynamic range and low cross-sensitivity; unpublished, using strategy from https://doi.org/10.1101/285866).
    Depositor
    Insert
    Tac promoter Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. C35m

    Plasmid
    #120969
    Purpose
    MoClo golden gate assembly CD part for RhlR (Rhl quorum-sensing receptor for C4-HSL, synonymously modified to remove internal BbsI and BsaI cut sites).
    Depositor
    Insert
    RhlR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI and BbsI sites removed; amino acid sequence …
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. C39m

    Plasmid
    #120972
    Purpose
    MoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    ccdB toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    BsaI site removed
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. C41m

    Plasmid
    #120974
    Purpose
    MoClo golden gate assembly CD part for dCas9 (Catalytically-inactive S. pyogenes Cas9, for gRNA-targeted repression). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    dCas9
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  10. C61m

    Plasmid
    #120977
    Purpose
    MoClo golden gate assembly CD part for RpaR* (improved RpaR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    RpaR from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. C62m

    Plasmid
    #120978
    Purpose
    MoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Insert
    TraR(W) from Scott and Hasty 2016
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. C64m

    Plasmid
    #120980
    Purpose
    MoClo golden gate assembly CD part for cI857 (temperature-sensitive lambda repressor that represses at 30C but not at 37C; see https://doi.org/10.1016/S0022-2836(66)80269-3. Use with P9m).
    Depositor
    Insert
    temperature sensitive lambda repressor (cI857)
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. C70m

    Plasmid
    #120982
    Purpose
    MoClo golden gate assembly CD part for RpaR-TYQEVV (Adam Meyer's improved RpaR activator selected for dynamic range and low cross-sensitivity using methods from https://doi.org/10.1101/285866,).
    Depositor
    Insert
    RpaR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. C71m

    Plasmid
    #120983
    Purpose
    MoClo golden gate assembly CD part for CinR-CIDDHYRTC n-2 (Adam Meyer's improved CinR activator from https://doi.org/10.1101/285866, selected for dynamic range and less cross-sensitivity).
    Depositor
    Insert
    CinR Adam Meyer
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 921 - 940 of 1937 results