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Plasmid#174627PurposeConstitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-383)-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
UseTagsGFP-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:ā¦PromoterChicken beta-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_mEGFP-DSep2_Peanut-Strep
Plasmid#174496Purposebacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila PeanutDepositorUseTagsStrep and mEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_R89A-PolyA
Plasmid#112291PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) R89A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Arginine 89 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_L91A-PolyA
Plasmid#112292PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) L91A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Leucine 91 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_F95A-PolyA
Plasmid#112293PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) F95A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationHuman YAP1-2gamma with Phenylalanine 95 to AlaninePromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H-Ras_V12-E38
Plasmid#39507DepositorInsertHRAS V12 E38 (HRAS Human)
UseTagsExpressionMammalianMutationG12V and D38EPromoterCMVAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-H-Ras_V12-A38
Plasmid#39513DepositorInsertHRAS V12 A38 (HRAS Human)
UseEukaryoticTagsExpressionMutationG12V and D38APromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSG5-H-Ras_V12-E38
Plasmid#39514DepositorInsertHRAS V12 E38 (HRAS Human)
UseEukaryoticTagsExpressionMutationG12V and D38EPromoterAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHyVec11
Plasmid#34794DepositorTypeEmpty backboneUseHydra expressionTagsIntergenic region between Hydra replication factoā¦ExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Supervillin-45
Plasmid#54263PurposeLocalization: Membrane/Actin, Excitation: 487, Emission: 509DepositorInsertSupervillin
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHyVec7
Plasmid#34793DepositorTypeEmpty backboneUseHydra expressionTagsGFP, RFP, and intergenic region between Hydra repā¦ExpressionMutationPromoterHydra vulgaris actin promoterAvailable sinceJan. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-iCre
Plasmid#72257PurposeExpress iCre under CB promoterDepositorInsertiCre
UseLentiviralTagsExpressionMammalianMutationPromoterCB promoter and chicken beta-actin + CMV enhancerAvailable sinceJan. 15, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-VASP-5
Plasmid#54296PurposeLocalization: Actin/Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertVASP
UseTagsmEmeraldExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-sEspin-C-18
Plasmid#54253PurposeLocalization: Actin Bundling, Excitation: 487, Emission: 509DepositorInsertsEspin
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEmerald-sEspin-N-18
Plasmid#54254PurposeLocalization: Actin Bundling, Excitation: 487, Emission: 509DepositorInsertsEspin
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRRLsin-SV40 T antigen-IRES-mCherry
Plasmid#58993Purposelentiviral expression of SV40 large T antigen and mCherry red fluorescent reporterDepositorInsertsCMV enhancer-chicken beta actin promoter
SV40 large T antigen
IRES-mCherry red fluorescent protein
UseLentiviralTagsExpressionMammalianMutationEcoRV site in MCS converted to BamHI sitePromoterCMV enhancer-chicken beta actinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-luciferase
Plasmid#55764Purposeluciferase reporter for in vivo bioluminescence imagingDepositorInsertCMV enhancer/chicken beta actin promoter
UseTagsExpressionMammalianMutationPromoterCMV enhancer/chicken beta actin promoterAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
1136- Desmoplakin-GFP
Plasmid#32227DepositorInsertDesmoplakin (DSP Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)
Plasmid#174631PurposeOpto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)DepositorInsertKIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Mouse, Synthetic)
UseTagsGFP-SSPB(micro)ExpressionMammalianMutationmmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ileā¦PromoterChicken beta-actinAvailable sinceNov. 3, 2021AvailabilityAcademic Institutions and Nonprofits only