Addgene

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XE118 CAMKII-T286D-CS2+

Plasmid

#16736

Depositor

Randall Moon

Article

Kühl et al J Biol Chem. 2000 Apr 28. 275(17):12701-

Insert

CAMKII-T286D (Camk2a Rat)

Use

Xenopus expression

Tags

Expression

Mutation

threonine 286 mutated to aspartate

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
OmEF1aCas9P2ApuroSB

Plasmid

#165484

Purpose

Stable expression of a zebrafish optimized Cas9 driven by a tilapia promoter in fish cells

Depositor

Dietmar Kueltz

Article

Hamar et al Sci Rep. 2021 Apr 12;11(1):7854. doi: 10

Insert

Zebrafish optimized Cas9 (nls-zcas9-nls from Wenbiao Chen Lab plasmid pCS2-nCas9n Addgene #47929)

Use

CRISPR; Transposon

Tags

Expression

Mutation

Promoter

Oreochromis mossambicus EF1 alpha promoter (OmEF1…

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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p4459 FLAG-hPLIC-1 NTF

Plasmid

#8664

Depositor

Peter Howley

Article

Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

Insert

hPLIC-1 (UBQLN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal fragment: amino acids 1-112 of hPLIC-1…

Promoter

Available since

June 16, 2005

Availability

Academic Institutions and Nonprofits only
HA-TRIM28deltaCC2

Plasmid

#124957

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Promoter

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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HS-OS-Plk4-mKate2

Plasmid

#105944

Purpose

heat shock inducible transgenesis, non phoshorylable version of Plk4

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

Plk4 (Plk4 Mouse)

Use

Tags

mKate2

Expression

Bacterial

Mutation

1002 to 3704 of Plk4/ deletion of S282-S305.

Promoter

Available since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

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HA-TRIM28deltaCC1

Plasmid

#124955

Purpose

Mammalian expression of Human TRIM28 with deleted CC domain

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of CC domains

Promoter

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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XE122 XDsh delta DIX-GFP-CS2+

Plasmid

#16787

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta DIX (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Expression

Mutation

deletion of aa 1-159

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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HS-aPKC-CAAX-GFP

Plasmid

#105946

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

Insert

aPKC (Prkcz Rat)

Use

Tags

CAAX and GFP

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad2 (100-467)

Plasmid

#14928

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (100-467) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

amino acids 100-467

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
CS2 Flag-Smad2 (1-185)

Plasmid

#14929

Depositor

Joan Massague

Article

Hata et al Nature. 1997 Jul 3. 388(6637):82-7.

Insert

Smad2 (1-185) (SMAD2 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

amino acids 1-185

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only
XE28 XBC 40

Plasmid

#16842

Depositor

Randall Moon

Article

Yost et al Genes Dev. 1996 Jun 15. 10(12):1443-54.

Insert

beta-catenin-myc (ctnnb1.L Frog)

Use

Xenopus expression

Tags

6x myc

Expression

Mutation

Promoter

Available since

May 22, 2009

Availability

Academic Institutions and Nonprofits only
Mad WT

Plasmid

#22996

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
Mad GSK3

Plasmid

#22995

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S208A, S204A

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
Mad MM

Plasmid

#22997

Depositor

Edward De Robertis

Article

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Use

Tags

FLAG

Expression

Mammalian

Mutation

S212A

Promoter

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
XE160 XDshHA-pt mutant PDZ-CS2+

Plasmid

#16780

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Expression

Mutation

point mutation in the binding pocket of the PDZ b…

Promoter

Available since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
XE161 XDshHA-triple pt mutant-CS2+

Plasmid

#16779

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

Dishevelled (dvl2.L Frog)

Use

Xenopus expression

Tags

HA

Expression

Mutation

triple point mutation outside of the PDZ binding …

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
acvr1l-VAAA-HA

Plasmid

#201905

Purpose

in vitro transcription of RNA encoding HA-tagged Acvr1l that contains 4 amino acid substitutions: T186V, S187A, S189A, S191A

Depositor

Mary Mullins

Article

Tajer et al Proc Natl Acad Sci U S A. 2021 Apr 13;11

Insert

acvr1l (acvr1l Zebrafish)

Use

Tags

HA

Expression

Mutation

T186V, S187A, S189A, S191A

Promoter

SP6

Available since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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XE155 XDpr-delta PDZB-CS2+

Plasmid

#16767

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

last four amino acids deleted

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
HA-14-3-3 zeta

Plasmid

#116888

Purpose

Expresses HA-tagged human 14-3-3 zeta in mammalian cells

Depositor

FENG-QIAN Li, Ken-Ichi Takemaru

Article

Li et al J Cell Biol. 2008 Jun 30;181(7):1141-54.

Insert

14-3-3 zeta (YWHAZ Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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mVenusC-Cavin3

Plasmid

#108890

Purpose

Expression of Cavin3 fused to the C-terminus of mVenus for bimolecular fluorescence complementation

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

Cavin3 (altered codon usage) (CAVIN3 Synthetic, Human)

Use

Tags

mVenus156-239

Expression

Mammalian

Mutation

altered codon usage

Promoter

Available since

March 23, 2019

Availability

Academic Institutions and Nonprofits only

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