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pFBD-Akt1(T308A/S473A)

Plasmid

#86578

Purpose

Expresses human full-length Akt1(T308A/S473A) phosphomutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

T308A/S473A phosphomutant

Promoter

Availability

Academic Institutions and Nonprofits only

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pFBD-Akt1(D323A/D325A)

Plasmid

#86592

Purpose

Expresses human full-length Akt1(D323A/D325A) mutant

Depositor

Ivan Yudushkin, Thomas Leonard

Article

Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

Insert

Akt1 (AKT1 Human)

Use

Tags

10xHis

Expression

Insect

Mutation

D323A/D325A transforming mutations

Promoter

Availability

Academic Institutions and Nonprofits only

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pGS_N_Strep

Plasmid

#101651

Purpose

Expression of type I signal-peptide containing proteins with N-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, KpnI linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pGS_C_Strep

Plasmid

#101652

Purpose

Expression of type I signal-peptide containing proteins with C-terminal Twin-Strep-tag in insect cells

Depositor

Bjoern Sander, Monika Golas

Article

Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

Insert

LRP6 Signal peptide, MCS linker, Twin-Strep tag

Use

Tags

Twin-Strep tag

Expression

Insect

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP1-Flag-His-WT

Plasmid

#111572

Purpose

Expresses full length PARP1 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP1 (PARP1 Human)

Use

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Mutation

Promoter

polyhedrin promoter

Availability

Academic Institutions and Nonprofits only

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pFastBac-LacI-LAP-HEC1

Plasmid

#114033

Purpose

Production of baculovirus with LacI-LAP-HEC1

Depositor

Geert Kops

Article

Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

Insert

LacI-LAP-HEC1 (NDC80 Human)

Use

Tags

Expression

Insect

Mutation

none

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG SMAD4

Plasmid

#83094

Purpose

Gateway entry vector for inducible expression of human SMAD4 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

SMAD4 (SMAD4 Human)

Use

Entry vector for gateway cloning

Tags

3xFLAG

Expression

Mutation

Promoter

TRE tight promoter

Availability

Academic Institutions and Nonprofits only
pDarmo.CMVT_v1

Plasmid

#133072

Purpose

Entry vector for cloning genes under an enhanced CMVT promoter for expression in mammalian cells. Version 1.0.

Depositor

David Sabatini, Kacper Rogala

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-Flag-TwinStrep and TwinStrep-Flag-3C

Expression

Mammalian

Mutation

Promoter

CMVT (Enhanced CMV)

Availability

Academic Institutions and Nonprofits only

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pDONRG_P4-P1R:MUM4_0.3Pro_35S

Plasmid

#128558

Purpose

Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloning

Depositor

George Haughn

Article

Dean et al Plant Mol Biol. 2017 Sep;95(1-2):33-50.

Type

Empty backbone

Use

Gateway promoter entry clone

Tags

Expression

Mutation

Promoter

MUM4 core promoter with 35S enhancer

Availability

Industry, Academic Institutions, and Nonprofits

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DYNC2LI1

Plasmid

#132527

Purpose

Human LIC3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNC2LI1 (DYNC2LI1 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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DYNLL1

Plasmid

#132530

Purpose

Human LC8-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL1 (DYNLL1 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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DYNLL2

Plasmid

#132531

Purpose

Human LC8-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLL2 (DYNLL2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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DYNLT3

Plasmid

#132532

Purpose

Human TCTEX-3, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLT3 (DYNLT3 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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DYNLRB1

Plasmid

#132533

Purpose

Human Rbl-1, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB1 (DYNLRB1 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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DYNLRB2

Plasmid

#132534

Purpose

Human Rbl-2, codon optimised for Sf9 expression.

Depositor

Andrew Carter

Article

Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

Insert

DYNLRB2 (DYNLRB2 Human)

Use

Tags

Expression

Insect

Mutation

Codon optimised for Sf9 expression

Promoter

Availability

Academic Institutions and Nonprofits only

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pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

Plasmid

#110560

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

stRBS-lacZ

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Availability

Academic Institutions and Nonprofits only

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pJM240 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-aacC1)

Plasmid

#110561

Purpose

miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-aacC1 insert

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

stRBS-aacC1

Use

Tags

Expression

Bacterial

Mutation

Promoter

rhaSR-PrhaBAD

Availability

Academic Institutions and Nonprofits only

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pACEBac1-CoRESTfl

Plasmid

#109156

Purpose

Encodes human full-length CoREST with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human full-length CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

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pACEBAC1-CoREST-C

Plasmid

#109159

Purpose

Encodes a human C-terminal CoREST fragment with C-terminal 3xFLAG tag to be expressed in a baculovirus/insect cell expression system

Depositor

Monika Golas

Article

Inui et al Protein Sci. 2017 May;26(5):997-1011. do

Insert

human C-terminal CoREST, sequence-optimized for insect cells (RCOR1 Human)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBacDual with LFn + Fla

Plasmid

#84866

Purpose

Baculovirus Expression of LFn -Fla fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

Depositor

Russell Vance

Article

Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

Inserts

LFn
Fla

Use

Baculovirus

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin

Availability

Academic Institutions and Nonprofits only

Enlarge